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Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3′ half and the 5′ terminus of virion RNA

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A Corrigendum to this article was published on 01 January 1978

Abstract

THE genome of Rous sarcoma virus (RSV) is a 30-40S RNA of 10,000 nucleotides coding for four known genes1 which map in the order 5′-gag, pol, env, src, poly(A)-3′ (refs 2, 3). This 30-40S RNA serves as mRNA for a gag precursor protein and perhaps a gag-pol precursor-polyprotein when translated in vitro4,5. The env gene, however, may be translated from a smaller intracellular messenger. Poly(A)-containing 20-24S cellular RNA from infected cells can, upon microinjection, complement an env-defective virus6 and may also direct in vitro synthesis of a protein that is serologically related to the env gene product7. Moreover, hybridisation with DNA complementary (cDNA) to env and src sequences of virion RNA detects discrete classes of subgenomic poly(A)-containing RNAs present in infected cells as steady state species, while gag and pol-specific cDNAs hybridise only to full sized RNA8,9.

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  1. Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California, 94720

    PAMELA MELLON & PETER H. DUESBERG

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  1. PAMELA MELLON
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  2. PETER H. DUESBERG
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MELLON, P., DUESBERG, P. Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3′ half and the 5′ terminus of virion RNA. Nature 270, 631–634 (1977). https://doi.org/10.1038/270631a0

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