Abstract
The primary structure of DNA containing the sequence for rat pituitary growth hormone mRNA has been determined. DNA was obtained by reverse transcription of polyadenylated RNA from cultured pituitary cells and from recombinant bacterial plasmids. The amino acid sequences for rat growth hormone and its precursor form have been deduced from the determined nucleotide sequences.
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References
Li, C. H. (ed.) Hormonal Proteins and Peptides 3 (Academic, New York, 1975).
Raiti, S. & Blizzard, R. M. Adv. Pediat. 17, 99–123 (1970).
Evans, H. M. & Li, C. H. Science 99, 183–184 (1944).
Guyda, H. J. & Friesen, H. G. Biochem. biophys. Res. Commun. 42, 1068–1075 (1971).
Wallis, M. Biochem. J. 125, 54P–56P (1971).
Niall, H. D. et al. Proc. natn. Acad. Sci. U.S.A. 68, 866–869 (1971).
Bewley, T. A., Dixon, J. S. & Li, C. H. Int. J. Peptide Prot. Res. 4, 281–287 (1972).
Li, C. H. et al. Experientia 24, 1288 (1968).
Seeburg, P. H. et al. Cell 12, 157–165 (1977).
Shine, J., Seeburg, P. H., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 494–499 (1977).
Tashjian, A. H., Jr et al. Endocrinology 82, 342 (1968).
Bancroft, F. C., Levine, L. & Tashjian, A. H., Jr J. Cell Biol. 43, 432–441 (1969).
Martial, J. A., Baxter, J. D., Goodman, H. M. & Seeburg, P. H. Proc. natn. Acad. Sci. U.S.A. 74, 1816–1820 (1977).
Blobel, G. & Sabatini, D. D. in Biomembranes 2 (ed. Manson, L. A.) 193–195 (Plenum, New York, 1971).
Bancroft, F. C. Expl Cell Res. 79, 275–278 (1973).
Sussman, P. M., Tushinski, R. J. & Bancroft, F. C. Proc. natn. Acad. Sci. U.S.A. 73, 29–33 (1976).
Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).
Wallis, M. & Davies, R. V. in Growth Hormone and Related Peptides (eds Pecile, A. & Muller, H. E.) 1–14 (Elsevier, New York, 1976).
Chantrenne, H., Burny, A. & Marbaix, G. Prog. Nucleic Acid Res. molec. Biol. 7, 173–194 (1967).
Palmiter, R. D. J. biol. Chem. 247, 6450–6459 (1972).
Duguid, J. R., Steiner, D. F. & Chick, W. L. Proc. natn. Acad. Sci. U.S.A. 73, 3539–3543 (1976).
Ullrich, A. et al. Science 196, 1313–1319 (1977).
Martial, J. A., Seeburg, P. H., Guenzi, D., Goodman, H. M. & Baxter, J. D. Proc. natn. Acad. Sci. U.S.A. (in the press).
Bancroft, F. C., Wu, G. & Zubay, G. Proc. natn. Acad. Sci. U.S.A. 70, 3646–3649 (1973).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Dayhoff, M. O. Atlas of Protein Sequence and Structure 5, Suppl. 2, 120–121 (National Biomedical Research Foundation, Washington, DC, 1976).
Baralle, F. E. Cell 10, 549–558 (1977).
Shine, J. et al. Proc. natn. Acad. Sci. U.S.A. 74, 1473–1477 (1977).
Efstratiadis, A., Kafatos, F. C., Maxam, A. M. & Maniatis, T. Cell 7, 279–288 (1976).
Sgamarella, V., van de Sande, J. H. & Khorana, H. G. Proc. natn. Acad. Sci. U.S.A. 67, 1468–1475 (1970).
Heynecker, H. L. et al. Nature 263, 748–752 (1976).
Scheller, R. H. et al. Science 196, 177–180 (1977).
Kornberg, A. Science 163, 1410–1418 (1969).
Bolivar, F. et al. Gene (in the press).
Maniatis, T., Kee, S. G., Efstradiadis, A. & Kafatos, F. C. Cell 8, 163–182 (1976).
Tushinski, R. J., Sussman, P. M., Yu, L. & Bancroft, F. C. Proc. natn. Acad. Sci. U.S.A. 74, 2357–2361 (1977).
Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).
Steitz, J. A. & Jakes, K. Proc. natn. Acad. Sci. U.S.A. 72, 4734–4738 (1975).
Shine, J. & Dalgarno, L. Biochem. J. 141, 609–615 (1974).
Efstratiadis, A., Kafatos, F. C. & Maniatis, T. Cell 10, 571–585 (1977).
Gralla, J. & Crothers, D. M. J. molec. Biol. 73, 497–511 (1973).
Thiele, D. et al. Molec. Biol. Rep. 1, 155–160 (1973).
Wilson, J. T. et al. Nucleic Acids Res. 7, 2353–2368 (1977).
Proudfoot, N. J. Cell 10, 559–570 (1977).
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Brawerman, G. A. Rev. Biochem. 42, 621–642 (1974).
Fiers, W. et al. Nature 260, 500–507 (1976).
Sanger, F. et al. Nature 265, 687–695 (1977).
Kirby, K. S. Biochim. biophys. Acta 36, 117–124 (1959).
Devillers-Thiery, A., Kindt, T., Scheele, G. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 72, 5016–5020 (1975).
Chan, S. J., Keim, P. & Steiner, D. F. Proc. natn. Acad. Sci. U.S.A. 73, 3539–3543 (1976).
Kemper, B. et al. Biochemistry 15, 15–19 (1976).
Schechter, I. & Burnstein, Y. Proc. natn. Acad. Sci. U.S.A. 73, 1964–1968 (1976).
Lingappa, V. R., Devillers-Thiery, A. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 74, 2432–2436 (1977).
Fellows, R. E. & Rogol, A. D. J. biol. Chem. 244, 1567–1575 (1969).
Laemmli, U. K. Nature 227, 680–685 (1970).
O'Farrell, P. Z., Huang, W. M. & Gold, L. M. J. biol. Chem. 248, 5499–5501 (1973).
Dingman, C. W. & Peacock, A. C. Biochemistry 7, 659 (1968).
Horiuchi, K. & Zinder, N. D. Proc. natn. Acad. Sci. U.S.A. 72, 2555–2558 (1975).
Vogt, V. M. Eur. J. Biochem. 33, 192–200 (1973).
Sugino, A. et al. J. biol. Chem. 252, 3987–3994 (1977).
Seeburg, P. H. & Schaller, H. J. molec. Biol. 92, 216–277 (1975).
Boyer, H. W. et al. Nature new Biol. 244, 40–43 (1973).
Jackson, D. A., Symons, R. H. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 69, 2904–2909 (1972).
Cohen, S. N., Chang, A. C. Y., Boyer, H. W. & Helling, R. B. Proc. natn. Acad. Sci. U.S.A. 70, 3240–3244 (1973).
Sharp, P. A., Sugden, B. & Sambrook, J. Biochemistry 12, 3055–3063 (1973).
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Seeburg, P., Shine, J., Martial, J. et al. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270, 486–494 (1977). https://doi.org/10.1038/270486a0
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DOI: https://doi.org/10.1038/270486a0
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