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Protonated polynucleotide structures

15. Metastable protonated polydeoxyribonucleotides

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Abstract

The CD spectra of three polydeoxyribonucleotide complexes, poly(dG-C). poly(dG-C), poly(dA-G). poly(dT-C) and poly(dA-C). poly(dT-G) have been studied as a function of pH at 25°C. Only poly(dA-G). poly(dT-C) showed large hysteresis, analogous to that observed for poly(dG). poly(dC), while the alternating pur-p-pyr containing polymers reversible titration curves exhibited. The hysteretic form R of poly(dA-G). poly(dT-C) is metastable at neutrality and reverts spontaneously and irreversibly to the neutral from N upon heating to about 60°. A whole titration cycle to pH 2.5 and back to neutrality has to be performed in order to obtain the metastable form R again.

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Abbreviations

CD:

circular dichroism

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Authors and Affiliations

  1. Service de Biochimie, Centre d'Études Nucléaires de Saclay, F-91190, Gif-sur-Yvette, France

    Danielle Thiele, Marie-Thérèse Sarocchi & Wilhelm Guschlbauer

  2. Abtl. Chemie, Max-Planck-Institut für experimentelle Medizin, D-3400, Göttingen, FRG

    Axel Lezius

  3. Groupe de Biophysique Moléculaire, Département de Biologie, Centre d'Études Nucléaires de Saclay, F-91190, Gif-sur-Yvette, France

    Christian Marck

Authors
  1. Danielle Thiele
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  2. Marie-Thérèse Sarocchi
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  3. Wilhelm Guschlbauer
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  4. Axel Lezius
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  5. Christian Marck
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Dedicated to Prof. A. Janke (Vienna) to his 85th birthday.

Part 14: this issue, p. 149.

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Thiele, D., Sarocchi, MT., Guschlbauer, W. et al. Protonated polynucleotide structures. Molecular Biology Reports 1, 155–160 (1973). https://doi.org/10.1007/BF00357156

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  • DOI: https://doi.org/10.1007/BF00357156

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