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Structure of an antibody combining site by magnetic resonance

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Abstract

The structure of the combining site of the DNP binding IgA mouse myeloma protein MOPC 315 has been determined by a combination of high resolution nuclear magnetic resonance, electron spin resonance, model building and chemical modifications. This approach yields the general dimensions of the site, its polarity and asymmetry features, the assignment of the DNP-contact residues and their three-dimensional coordinates.

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Authors and Affiliations

  1. The Department of Biochemistry, Oxford University, UK

    R. A. Dwek, S. Wain-Hobson, S. Dower, P. Gettins, B. Sutton & S. J. Perkins

  2. The Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

    D. Givol

Authors
  1. R. A. Dwek
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  2. S. Wain-Hobson
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  3. S. Dower
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  4. P. Gettins
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  5. B. Sutton
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  6. S. J. Perkins
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  7. D. Givol
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Dwek, R., Wain-Hobson, S., Dower, S. et al. Structure of an antibody combining site by magnetic resonance. Nature 266, 31–37 (1977). https://doi.org/10.1038/266031a0

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  • DOI: https://doi.org/10.1038/266031a0

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