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Nonsense suppressors of Saccharomyces cerevisiae can be generated by mutation of the tyrosine tRNA anticodon

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Abstract

The yeast amber suppressor, SUP5-a, was previously shown to cause the insertion of tyrosine at the sites of UAG nonsense codons. Nucleotide sequencing established that this SUP5-a suppressor specifies a mutant tyrosine transfer RNA (tRNA) which has the anticodon CΨA instead of the normal GΨA, an alteration identical to that found in the tyrosine-inserting amber suppressor from Escherichia coli.

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Author notes

  1. Peter W. Piper

    Present address: Imperial Cancer Research Fund Laboratories, Lincolns Inn Fields, London, WC2 3PX, UK

Authors and Affiliations

  1. Department of Molecular Biology, University of Aarhus, 8000, Aarhus, Denmark

    Peter W. Piper & Marilyn Wasserstein

  2. Department of Biostructural Chemistry, University of Aarhus, 8000, Aarhus, Denmark

    Frode Engbaek, Keld Kaltoft & Julio E. Celis

  3. Department of Human Genetics, University of Aarhus, 8000, Aarhus, Denmark

    Jesper Zeuthen

  4. Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642

    Susan Liebman & Fred Sherman

Authors
  1. Peter W. Piper
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  2. Marilyn Wasserstein
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  3. Frode Engbaek
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  4. Keld Kaltoft
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  5. Julio E. Celis
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  6. Jesper Zeuthen
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  7. Susan Liebman
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  8. Fred Sherman
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Piper, P., Wasserstein, M., Engbaek, F. et al. Nonsense suppressors of Saccharomyces cerevisiae can be generated by mutation of the tyrosine tRNA anticodon. Nature 262, 757–761 (1976). https://doi.org/10.1038/262757a0

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  • DOI: https://doi.org/10.1038/262757a0

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