Skip to main content
SpringerLink
Log in

Relationship between tritiated poly-L-lysine binding and template activity of human chromosomes

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

IT has been reported that cytological preparations of chromosomes can serve as templates in RNA polymerase reactions1–2. The newly synthesised RNA on chromosomes was readily digested with RNase in contrast to RNA which was synthesised in a similar manner but using as a template denatured DNA immobilised on nitrocellulose filters1. When cytological preparations of chromosomes were pretreated with poly-L-lysine (PL), no synthesis of RNA could be demonstrated2. The implication was that bound PL blocked those sites on DNA template which were responsible for transcription.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sederoff, R., Clynes, R., Poncz, M., and Hachtel, S., J. Cell Biol., 57, 538 (1973).

    Article  CAS  Google Scholar 

  2. Umiel, N., and Plaut, W., J. Cell Biol., 56, 139 (1973).

    Article  CAS  Google Scholar 

  3. Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. M., and Hungerford, D. A., Exp Cell Res., 20, 613 (1960).

    Article  CAS  Google Scholar 

  4. Evans, H. J., Br. med. Bull., 29, 196 (1973).

    Article  CAS  Google Scholar 

  5. Caspersson, T., Zech, L., Johansson, C., and Modest, E. J., Chromosoma, 30, 215 (1970).

    Article  CAS  Google Scholar 

  6. Du Praw, E. J., DNA and Chromosomes, 135 (Holt, Rinehart and Winston, New York, 1970).

  7. Hsu, T. C., Mammal. Chromosome Newsl., 13, 43 (1972).

    Google Scholar 

  8. Seabright, M., Lancet, ii, 971 (1971).

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, State University of New York at Albany, Albany, New York, 12222

    S. P. MARFEY & M. G. LI

Authors
  1. S. P. MARFEY
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. G. LI
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

MARFEY, S., LI, M. Relationship between tritiated poly-L-lysine binding and template activity of human chromosomes. Nature 249, 559–561 (1974). https://doi.org/10.1038/249559a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/249559a0

  • Springer Nature Limited

This article is cited by

Search

Navigation