Skip to main content
SpringerLink
Log in

Latex spheres as markers for studies of cell surface receptors by scanning electron microscopy

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

THE distribution of antigens and carbohydrate residues on the surfaces of cells, notably erythrocytes and lymphocytes, can be determined by binding antibodies or lectins to such macromolecules as ferritin1,2, haemocyanin3,4 or peroxidase2,5, which serve as markers for transmission electron microscopy. Improved techniques in high resolution scanning electron microscopy (SEM), however, make it possible to determine the topographical distribution of molecular receptors on the surfaces of cells and tissues by similar histochemical techniques involving markers resolvable by SEM. LoBuglio et al.6 used commercial polystyrene latex particles, 2,300Å in diameter, as immunological markers for SEM work. But applications of this reagent are limited because the hydrophobic surface of the particles makes them stick non-specifically to many surfaces and molecules.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nicolson, G. L., Masouredis, S. P., and Singer, S. J., Proc. natn. Acad. Sci. U.S.A., 68, 1416 (1971).

    Article  ADS  CAS  Google Scholar 

  2. Wagner, M., Res. Immunochem. Immunobiol., 3, 185 (1973).

    CAS  Google Scholar 

  3. Smith, S. B., and Revel, J.-P., Devl. Biol., 27, 434 (1972).

    Article  CAS  Google Scholar 

  4. Karnovsky, M., and Unanue, E. R., Fedn. Proc., 32, 55 (1973).

    CAS  Google Scholar 

  5. Avrameas, S., Int. Rev. Cytol., 27, 349 (1970).

    Article  CAS  Google Scholar 

  6. LoBuglio, A. F., Rinehart, J. J., and Balcerzak, S. P., in Scanning Electron Microscopy, Part II, 313 (ITT Research Institute, Chicago, 1972).

    Google Scholar 

  7. Wichterle, O., in Encylopedia Polymer Sci. Tech., 15, 27B (Interscience, New York, 1971).

    Google Scholar 

  8. Cuatrecasas, P., J. biol. Chem., 245, 3059 (1970).

    CAS  Google Scholar 

  9. Hoffman, A. S., Schmer, G., Harris, C., and Kraft, W. G., Trans. Am. Soc. artif. int. Organs, 18, 10 (1972).

    Article  CAS  Google Scholar 

  10. Goodfriend, T. L., Levine, L., and Fasman, G., Science, 144, 1355 (1964).

    Article  Google Scholar 

  11. Bessis, M., and Weed, R. I., in Scanning Electron Microscopy, Part II, 289 (ITT Research Institute, Chicago, 1972).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91109

    ROBERT S. MOLDAY & WILLIAM J. DREYER

  2. Jet Propulsion Laboratory, Pasadena, California, 91103

    ALAN REMBAUM & S. P. S. YEN

Authors
  1. ROBERT S. MOLDAY
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. WILLIAM J. DREYER
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. ALAN REMBAUM
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. P. S. YEN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

MOLDAY, R., DREYER, W., REMBAUM, A. et al. Latex spheres as markers for studies of cell surface receptors by scanning electron microscopy. Nature 249, 81–83 (1974). https://doi.org/10.1038/249081a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/249081a0

  • Springer Nature Limited

This article is cited by

Search

Navigation