Abstract
A NEW approach to the study of nucleic acid structure lies in the investigation of infrared spectra in the 950–1,350 cm−1 range where the main bands represent the sugar-phosphate backbone vibrations1. In principle, water transmittance in this region enables spectra of aqueous solutions to be obtained2 and the absence of strong protein bands provides a unique opportunity for investigating nucleic acid structure inside the nucleoprotein particles. We have carried out temperature-dependent measurements of DNA and RNA spectra both in the free state and within viruses. Our results show that the spectra reflect accurately the peculiarities of the sugar-phosphate skeleton of nucleic acids.
Similar content being viewed by others
References
Tsuboi, M., Appl. Spectr. Rev., 3, 45 (1969).
Tsuboi, M., Progr. Theor. Phys., Suppl. No. 17, 99 (1961).
Tikhonenko, T. I., Velikodvorskaya, G. A., and Zemtsova, E. V., Biokhimiya (USSR), 27, 726 (1962).
Shie, M., Chirgadze, Yu. N., and Tikhonenko, T. I., Voprosy Virusologii (USSR), 5, 619 (1970).
Tikhonenko, T. I., Dobrov, E. N., Velikodvorskaya, G. A., and Kiseleva, N., J. Mol. Biol., 18, 58 (1966).
Tikhonenko, T. I., Adv. Virus Res., 15, 101 (1969).
Sato, T., et al., J. Mol. Biol., 16, 180 (1966).
Brahms, J., Maurizot, J. C., and Pilet, J., Biochim. Biophys. Acta, 186, 110 (1969).
Klug, A., and Caspar, D., Adv. Virus Res., 7, 225 (1960).
Dobrov, E., and Kust, S., Voprosy Virusologii (USSR), 3, 366 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SHIE, M., KHARITONENKOV, I., TIKHONENKO, T. et al. New Possibilities of Investigating Nucleic Acids and Nucleoproteins in Aqueous Solutions by Infrared Spectroscopy. Nature 235, 386–388 (1972). https://doi.org/10.1038/235386a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/235386a0
- Springer Nature Limited
This article is cited by
-
IR spectra of aqueous solutions of some polymers
Journal of Applied Spectroscopy (1978)
-
IR spectroscopy of mucopolysaccharides in aqueous solutions
Journal of Applied Spectroscopy (1977)