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Transformation and Virus Growth by Murine Sarcoma Viruses in Human Cells

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Abstract

IN the study of neoplastic transformation of human cells in culture, the quantitative systems so far described have used only the DNA-containing tumour viruses SV40 (ref. 1) and adenovirus type 12 (ref. 2). Various factors, both genetic3 and environmental4, influence the efficiency of transformation with these viruses. We describe here a quantitative transformation system using an RNA-containing tumour virus, murine sarcoma virus (MSV)5. The transformation of human cells by MSV is inefficient, but in optimal conditions (logarithmically growing cells and certain highly susceptible cell strains) both cell transformation and virus growth can be demonstrated.

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References

  1. Aaronson, S. A., and Todaro, G. J., Virology, 36, 254 (1968).

    Article  CAS  PubMed  Google Scholar 

  2. Todaro, G. J., and Aaronson, S. A., Proc. US Nat. Acad. Sci., 61, 1272 (1968).

    Article  ADS  CAS  Google Scholar 

  3. Todaro, G. J., Green, H., and Swift, M. R., Science, 153, 1252 (1966).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Pollock, E. J., and Todaro, G. J., Nature, 219, 520 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Huebner, B. J., Proc. US Nat. Acad. Sci., 58, 835 (1967).

    Article  ADS  CAS  Google Scholar 

  6. Todaro, G. J., and Aaronson, S. A., Virology, 38, 174 (1969).

    Article  CAS  PubMed  Google Scholar 

  7. Klement, V., Hartley, J. W., Rowe, W. P., and Huebner, R. J., J. Nat. Cancer Inst. (in the press).

  8. Bernhard, W., Cancer Res., 20, 712 (1960).

    CAS  PubMed  Google Scholar 

  9. Biswal, N., Grizzard, M. B., McCombs, R. M., and Benyesh-Melnick, M., J. Virol., 2, 1346 (1968).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Stenkvist, B., and Ponten, J., Acta Path. Microb. Scand., 62, 315 (1964).

    Article  CAS  PubMed  Google Scholar 

  11. Bernard, C., Lasneret, J., Boucher, M., and Boiron, M., CR Acad. Sci., D 268, 624 (1969).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland, 20014

    STUART A. AARONSON & GEORGE J. TODARO

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  1. STUART A. AARONSON
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  2. GEORGE J. TODARO
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AARONSON, S., TODARO, G. Transformation and Virus Growth by Murine Sarcoma Viruses in Human Cells. Nature 225, 458–459 (1970). https://doi.org/10.1038/225458a0

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  • DOI: https://doi.org/10.1038/225458a0

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