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Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain

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Abstract

IT is well established that when brain tissue is homogenized large numbers of nerve terminals pinch off to form intact membrane-bound particles known as “synaptosomes”. These can be isolated from brain homogenates by density gradient centrifugation1–3. Such particles contain various presumptive transmitter substances such as acetylcholine, noradrenaline (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT)1–3. Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established. Attempts to separate synaptosomes storing different transmitters, however, have met with only limited success4–6.

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Author notes

  1. LESLIE L. IVERSEN

    Present address: Department of Pharmacology, University of Cambridge, Cambridge, England

Authors and Affiliations

  1. Departments of Pharmacology and Experimental Therapeutics and Psychiatry and the Behavioral Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205

    LESLIE L. IVERSEN & SOLOMON H. SNYDER

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  1. LESLIE L. IVERSEN
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  2. SOLOMON H. SNYDER
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IVERSEN, L., SNYDER, S. Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain. Nature 220, 796–798 (1968). https://doi.org/10.1038/220796a0

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  • DOI: https://doi.org/10.1038/220796a0

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