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Relationship between pH and Electrophoretic Mobility for Lymphocytes circulating in Chronic Lymphocytic Leukaemia

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Abstract

THERE is a surprising lack of electrokinetic data about human lymphocytes circulating in healthy and diseased people. We have attempted the electrokinetic characterization of lymphocytes circulating in a patient with chronic lymphocytic leukaemia, principally because lymphocytes from certain people with this disorder had been reported, in certain conditions, to possess adhesiveness for polystyrene beads different from normal lymphocytes. The electrophoretic mobilities of saline-washed lymphocytes obtained from this patient (case 1 first investigated2) were determined at 25° C in the pH range 1.9 to 10.7 with a constant ionic strength of saline (0.145 M). The relationship between pH and electrophoretic mobility suggests that the electrokinetic behaviour of the lymphocytes arose as a result of the presence of several ionizable (acidic and basic) groups on the surface.

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References

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Authors and Affiliations

  1. Department of Radiotherapeutics, University of Cambridge, Addenbrooke's Hospital, Trumpington Street, Cambridge

    J. N. MEHRISHI

  2. Cytochemistry Section, Division of Chemistry and Biochemistry, Imperial Cancer Research Fund, Lincoln's Inn Fields, London

    A. E. R. THOMSON

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  1. J. N. MEHRISHI
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  2. A. E. R. THOMSON
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MEHRISHI, J., THOMSON, A. Relationship between pH and Electrophoretic Mobility for Lymphocytes circulating in Chronic Lymphocytic Leukaemia. Nature 219, 1080–1081 (1968). https://doi.org/10.1038/2191080a0

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  • DOI: https://doi.org/10.1038/2191080a0

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