Abstract
Exosomes are cell-derived vesicles that have been implicated in the pathogenesis of many inflammatory diseases. In the immune system, it has been shown that T lymphocyte-derived exosomes are able to induce diverse cellular responses. There are several methods to isolate and to characterize exosomes, each with their own advantages and disadvantages. Here, we describe a centrifugation approach, combined with mass spectrometry characterization, as a means to study exosomes derived from primary human T lymphocytes. This method is sensitive and therefore can be applied when a limited amount of sample is available.
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Acknowledgments
This work was supported by the National Institute of Health (R01 AI125640) and Cancer Research Institute grants to A.M.
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Azoulay-Alfaguter, I., Mor, A. (2020). Isolation and Characterization of T Lymphocyte-Exosomes Using Mass Spectrometry. In: Mishra, S. (eds) Immunometabolism. Methods in Molecular Biology, vol 2184. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0802-9_7
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DOI: https://doi.org/10.1007/978-1-0716-0802-9_7
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