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Evidence for binding of Cytoplasmic Creatine Kinase to Structural Elements in Heart Muscle

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Abstract

THERE have been suggestions1,2 that cytoplasmic creatine kinase is not always free in solution in the cell, but that it is in part bound to structural elements, for example myofibrils. Some evidence for this is provided inter alia by localization studies3–5. I have obtained direct evidence for the binding of some cytoplasmic creatine kinase by isolation and crystallization of the enzyme from the myofibrillar fraction of a heart muscle homogenate. The enzyme was differentiated from the mitochondrial creatine kinase, which forms about 50 per cent of the total creatine kinase activity in heart muscle6.

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Authors and Affiliations

  1. Department of Biochemistry, University of Edinburgh Medical School,

    J. H. OTTAWAY

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  1. J. H. OTTAWAY
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OTTAWAY, J. Evidence for binding of Cytoplasmic Creatine Kinase to Structural Elements in Heart Muscle. Nature 215, 521–522 (1967). https://doi.org/10.1038/215521b0

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