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Histochemical Localization of Intestinal Disaccharidases: Application to Peroral Biopsy Specimens

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Abstract

THIS communication describes a technique for localizing intestinal disaccharidases developed from that of Dahlqvist and Brun1. Intestinal biopsies were obtained by a multipurpose suction biopsy tube2 from four normal children and five patients with sucrose and isomaltose “intolerance”. Intestinal specimens from rats and guinea-pigs have also been investigated by the same procedure. The biopsies and animal tissues were rapidly frozen and stored at −25°. Sections from SJJL were cut in a cryostat, mounted on coverslips and fixed in cold acetone for 15 min.

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References

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Authors and Affiliations

  1. Clinique et Unité de Recherches de Génétique Médicale, Hôpital des Enfants-Malades, 149 Rue de Sévres, Paris

    J. JOS, J. FRÉZAL, J. REY & M. LAMY

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  1. J. JOS
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  2. J. FRÉZAL
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  3. J. REY
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  4. M. LAMY
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JOS, J., FRÉZAL, J., REY, J. et al. Histochemical Localization of Intestinal Disaccharidases: Application to Peroral Biopsy Specimens. Nature 213, 516–518 (1967). https://doi.org/10.1038/213516a0

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