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Effect of Mitomycin C on Early Phenotypic Expression in the Transformation of Diplococcus pneumoniae

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Abstract

CONCERNING the transcription of genetic information by DNA, recent investigations, using systems of the transformation of D. pneumoniae1 and infection with some bacteriophages2–4, have demonstrated that the DNA molecule consists of an informational and a non-informational strand. Guild and Robinson1 succeeded in obtaining fractions of denatured transforming DNA in CsCl density-gradient centrifugation which seemed to correspond to each polynucleotide chain, and they examined the time course of the appearance of cells possessing the new phenotype. It was observed that the expression of the new phenotype by one fraction was retarded for about one generation period compared with the other fraction. The messenger RNA synthesized after infection with bacteriophages2,3, or the RNA synthesized enzymatically in an in vivo system2,4, were examined for hybrid formation with polynucleotide chains separated in CsCl density-gradient centrifugation. The data suggested that the messenger RNA produced in vivo can form a ribonuclease-resistant hybrid with the unique polynucleotide chain of the primer DNA.

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References

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Authors and Affiliations

  1. Laboratory of Biochemistry, National Institute of Radiological Sciences, Anagawa, Chiba City, Japan

    KENSHI SUZUKI, HIROKO YAMAGAMI & YOSHIE SHIMAZU

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  1. KENSHI SUZUKI
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  2. HIROKO YAMAGAMI
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  3. YOSHIE SHIMAZU
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SUZUKI, K., YAMAGAMI, H. & SHIMAZU, Y. Effect of Mitomycin C on Early Phenotypic Expression in the Transformation of Diplococcus pneumoniae. Nature 205, 929–930 (1965). https://doi.org/10.1038/205929a0

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