Skip to main content
SpringerLink
Log in

Respiratory Chain-linked Lactic Oxidase of Bakers' Yeast

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

VARIOUS forms of lactic dehydrogenase (not iso-enzymes) are reported in yeast1. Apart from one specific for D(−) lactate in the soluble constituents of the yeast cell, Morton observed that there exists in the particulate fraction of anaerobic yeast a lactic dehydrogenase which is a flavoprotein (free from hæem) specific for D(−) lactate whereas the enzyme in the particles of aerobic yeast, the hæmo-flavoprotein, is specific for L(+) lactate2. The D (−) lactic dehydrogenase has been purified and studied by Nygaard3 and Gregolin and Singer4. The physiological significance, however, of lactic dehydrogenase or lactic cytochrome c reductase has not been found. Gregolin and Singer4 could not find oxygen consumption in particles of aerobic yeast with D(−) lactate as substrate unless external cytochrome c had been added, and hence concluded that the enzyme is not directly linked to the cytochrome system of yeast, at least in the respiratory particle preparation. This note, however, reports a lactic oxidase in a preparation of mitochondrial particles from aerobic bakers' yeast (Saccharomyces cerevisiae) oxidizing D(−) lactate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nygaard, A. P., in Wright, B., ed., Control Mechanisms in Respiration and Fermentation, 27 (Ronald Press Co., New York, 1963). Singer, T. P., Gregolin, C., and Cremona, T., ibid., 47. Nygaard, A. P., in Haematin Enzymes, edit. by Falk, J. E., Lemberg, R., and Morton, R. K., 544 (Pergamon Press, 1961).

    Google Scholar 

  2. Morton, R. K., ibid., 233.

  3. Nygaard, A. P., J. Biol. Chem., 236, 920, 2128 (1961).

    CAS  PubMed  Google Scholar 

  4. Gregolin, C., and Singer, T. P., Biochim. Biophys. Acta, 67, 201 (1963).

    Article  CAS  Google Scholar 

  5. Chance, B., in Methods of Enzymology, edit. by Colowick, S. P., and Kaplan, N. O., 4, 273 (Academic Press, New York).

  6. Chance, B., and Baltscheffsky, M., Biochem. J., 68, 283 (1958).

    Article  CAS  Google Scholar 

  7. Chapell, J. B., in Goodwin, T. W., and Lindberg, O., editors, Biological Structure and Functions, 71 (Academic Press, New York).

Download references

Author information

Author notes

  1. B. R. ROY

    Present address: Central Food Laboratory, 3 Kyd Street, Calcutta

Authors and Affiliations

  1. Johnson Research Foundation, Department of Biophysics and Physical Biochemistry, University of Pennsylvania, Philadelphia

    B. R. ROY

Authors
  1. B. R. ROY
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

ROY, B. Respiratory Chain-linked Lactic Oxidase of Bakers' Yeast. Nature 201, 80–81 (1964). https://doi.org/10.1038/201080a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/201080a0

  • Springer Nature Limited

This article is cited by

Search

Navigation