Abstract
I HAVE succeeded1 in keeping alive living materials from the cell interiors of which all the easily freezable water was drawn by sufficient extra-cellular freezing at −30° C., after immersion in liquid nitrogen.
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References
Sakai, A., Low Temp. Sci., Ser. B, 14, 17 (1956).
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Asahina, E., and Aoki, K., Nature, 182, 327 (1958).
Asahina, E., Low Temp. Sci., Ser. B, 16, 65 (1958).
Asahina, E. (unpublished).
Nei, T. (unpublished).
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SAKAI, A. Survival of the Twig of Woody Plants at −196° C.. Nature 185, 393–394 (1960). https://doi.org/10.1038/185393a0
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DOI: https://doi.org/10.1038/185393a0
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