Abstract
IN the course of an investigation on the geographical distribution of the Azotobacteriaceae1 in various types of soil in India, a nitrogen-fixing organism has been isolated from a sample of red soil from Pattambi (Madras) by Winogradsky's silica-gel plate technique and purified by dilution platings. This new bacterium differs in certain morphological and physiological characteristics from known species of Azotobacter (including Beijerinckia)1. The cells are oval in shape, occur mostly in pairs and packets and sometimes singly, measuring on an average 1.2–2.2µ × 2.3–4.6µ (Fig. 1). The cells do not change their shape even on prolonged incubation in Ashby's nitrogen-free liquid medium with different carbohydrates. The bacterium encysts after 30 days incubation at 30° C. in the solid mannitol medium without calcium carbonate. The optimum temperature for growth was found to be about 30° C.
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References
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ROY, A. A New Species of Azotobacter producing Heavy Slime and Acid. Nature 182, 120–121 (1958). https://doi.org/10.1038/182120a0
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DOI: https://doi.org/10.1038/182120a0
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