Abstract
Azotobacter is a renowned nonsymbiotic nitrogen fixer. Since the discovery of Azotobacter in 1901, it has magnetized microbiologists’ attention for its interesting potential in agriculture for nitrogen fixation as well as synthesis of biologically active substances. It has distinctly enhanced effect on crop production in agriculture by which it deciphers the growing demand of food for ever-increasing population. The exploration of free-living Azotobacter sp. along with unique symbiotic A. paspali will be an attempt towards augmentation of soil fertility with enhanced crop yield. This chapter will brief the general strategy for isolation and identification of Azotobacter with elementary approach.
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Acknowledgments
We acknowledge Prof. Ninfa Rosas-García, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Mexico for inspiring agrobiotech work and moral support. Author BVM is thankful to Principal, Bajaj College of Science, Wardha for the support and encouragement.
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Mohite, B.V., Patil, S.V. (2022). Isolation and Identification of Nonsymbiotic Azotobacter and Symbiotic Azotobacter Paspali–Paspalum notatum. In: Amaresan, N., Patel, P., Amin, D. (eds) Practical Handbook on Agricultural Microbiology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1724-3_3
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DOI: https://doi.org/10.1007/978-1-0716-1724-3_3
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