Abstract
Two difficulties are frequently encountered when it is desired to grow pathogenic bacteria with active aeration, namely, foaming and wetting of the exhaust filter. Chemical or mechanical foam-breaking and heating systems can be used to avoid these problems but have many disadvantages. I have used them mainly on large-scale equipment1,2. However, it was found that a basically different approach had to be used for ordinary laboratory equipment. Since the method which has been developed may have wide applications, it will be described below.
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Chain, E. B., Paladino, S., Callow, D. S., Ugolini, F., and Van der Sluis, J., Bull. World Health Org., 6, 73 (1952).
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HEDÉN, CG. Pulsating Aeration of Microbial Cultures. Nature 179, 324–325 (1957). https://doi.org/10.1038/179324b0
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DOI: https://doi.org/10.1038/179324b0
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