Abstract
We have developed a laser-based printing technique, called biological laser printing (BioLP™). BioLP is a non-contact, orifice-free technique that rapidly deposits fL to nL scale volumes of biological material with spatial accuracy better than 5 μm. The printer's orifice-free nature allows for transfer of a wide range of biological material onto a variety of substrates. Control of transfer is performed via a computer-aided design/computer-aided manufacturing (CAD/CAM) system which allows for deposition rates up to 100 pixels of biological material per second using the current laser systems. In this article, we present a description of the apparatus, a model of the transfer process, and a comparison to other biological printing techniques. Further, examples of current system capabilities, such as adjacent deposition of multiple cell types, large-scale cell arrays, and preliminary experiments on creating multi-layer cell constructs are presented. These cell printing experiments not only demonstrate near 100% viability, they also are the first steps toward using BioLP to create heterogeneous 3-dimensional constructs for use in tissue engineering applications.
Similar content being viewed by others
References
J.A. Barron, B.J. Spargo, and B.R. Ringeisen, Applied Physics A, in press (2004).
S. Belkin, Current Opinions in Microbiology 6, 206 (2003).
S. Belkin, T.K. Van Dyk, A.C. Vollmer, D.R. Smulski, and R.A. LaRossa, Environmental Toxicology and Water Quality 11, 179 (1996).
A. Bruckbauer, L.M. Ying, A.M. Rothery, D.J. Zhou, A.I. Shevchuk, C. Abell, Y.E. Korchev, and D. Klenerman, Journal of the American Chemical Society 124, 8810 (2002).
M.V. Catani, A. Rossi, A. Costanzo, S. Sabatini, M. Levrero, G. Melino, and L. Avigliano, Biochemical Journal 356, 77 (2001).
M.S. Chapekar, Journal of Biomedical Materials Research 53, 617 (2000).
Y. Dou, L.V. Zhigilei, A. Postawa, N. Winograd, and B.J. Garrison, Nuclear Instruments and Methods in Physics Research B 180, 105 (2001).
H.A. Fishman, O. Orwar, R.H. Scheller, and R.N. Zare, Proceedings of the National Academy of Sciences of the United States of America 92, 7877 (1995).
L.G. Griffith and G. Naughton, Science 295, 1009 (2002).
P. Gwynne and G. Heebner, Drug Discovery and Biotechnology Trends: Proteomics 2: Microarrays, the Next Step, Science E-Marketplace, http://www.sciencemag.org/feature/e-market/benchtop/ddbt_10303.shl (2003a).
P. Gwynne and G. Heebner, Drug Discovery and Biotechnology Trends: Analysis and Separation: DNA and Biochips 1, Science E-Marketplace, http://www.sciencemag.org/feature/e-market/benchtop/ddbd_0314.shl (2003b).
J. Hyun, S.J. Ahn, W.K. Lee, A. Chilkoti, and S. Zauscher, Nanoletters 2, 1203 (2002).
R.S. Kane, S. Takayama, E. Ostuni, D.E. Ingber, and G.M. Whitesides, Biomaterials 20, 2363 (1999).
S. Kohler, S. Belkin, and R.D. Schmid, Fresenius Journal of Analytical Chemistry 366, 769 (2000).
K.B. Lee, J.H. Lim, and C.A. Mirkin, Journal of the American Chemical Society 125, 5588 (2003).
K.B. Lee, S.J. Park, C.A. Mirkin, J.C. Smith, and M. Miksich, Science 295, 1702 (2002).
W. Malomi, E. Straface, G. Donelli, and P.U. Giacomoni, European Journal of Dermatology 6, 414 (1996).
V. Mironov, T. Boland, T. Trusk, G. Forgacs, and R.R. Markwald, Trends in Biotechnology 21, 157 (2003).
E. Palik, Handbook of Optical Constants of Solids (Academic Press, New York, 1985).
W.H. Parkinson and K. Yoshino, Chemical Physics 294, 31 (2003).
B.R. Ringeisen, J.A. Barron, and D.B. Krizman, in Protein Microarrays, edited by P. Schena (Jones and Bartlett, Boston, 2004).
B.R. Ringeisen, H. Kim, J.A. Barron, D.B. Krizman, D.B. Chrisey, S. Jackman, R.Y.C. Anyeung, and B.J. Spargo, Tissue Engineering, in press (2004).
B.R. Ringeisen, P.K. Wu, H. Kim, A. Pique, R.Y.C. Auyeung, H.D. Young, and D.B. Chrisey, Biotechnology Progress 18, 1126 (2002).
A. Roda, M. Guardigli, C. Russo, P. Pasini, and M. Baraldini, Biotechniques 28, 492 (2000).
R.S. Takayama, E. Ostuni, D.E. Ingber, and G.M. Whitesides, Biomaterials 20, 2363 (1999).
C. Vedrine, J.C. Leclerc, C. Durrieu, and C. Tran-Minh, Biosensors and Bioelectronics 18, 457 (2003).
A.C. Vollmer, S. Belkin, D.R. Smulski, T.K. Van Dyk, and R.A. LaRossa, Applied and Environmental Microbiology 63, 2566 (1997).
W.C Wilson and T. Boland, Anatomical Record Part A-Discoveries in Molecular, Cellular and Evolutionary Biology 272A, 491 (2003).
P.K Wu, B.R. Ringeisen, D.B. Krizman, C.G. Frondoza, M. Brooks, D.M. Bubb, R.C.Y. Auyeung, A. Pique, B. Sparge, R.A. McGill, and D.B. Chrisey, Review of Scientific Instruments 74, 2546 (2003).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Barron, J., Wu, P., Ladouceur, H. et al. Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns. Biomedical Microdevices 6, 139–147 (2004). https://doi.org/10.1023/B:BMMD.0000031751.67267.9f
Issue Date:
DOI: https://doi.org/10.1023/B:BMMD.0000031751.67267.9f