Abstract
The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% ± 7% on Matrigel. Lipid volume surpassed 4.5×103 μm3 cell−1 for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3×103 μm2 cell−1. These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alster TS, West TB (2000) Human-derived and new synthetic injectable materials for soft-tissue augmentation: current status and role in cosmetic surgery. Plast. Reconstr. Surg. 105: 2515–2525.
Hauner H, Entenmann G, Wabitsch M, Gaillard D, Aihaud G (1989) Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium. Clin. Invest. 84: 1663–1670.
Hausman GJ, Wright JT, Richardson RL (1996) The influence of extracellular matrix substrata on preadipocyte development in serum-free cultures of stromal-vascular cells. J. Anim. Sci. 74: 2117–2128.
Katz AJ, Llull R, Hedrick MH, Futrell JW (1999) Emerging approaches to the tissue engineering of fat. Clin. Plast. Surg. 26: 587–602.
Kral JG, Crandall DL (1999) Development of a human adipocyte synthetic polymer scaffold. Plast. Reconstr. Surg. 104: 1732–1738.
Kubo Y, Kaidzu S, Nakajima I, Takenouchi K, Naksmura F (2000) Organization of extracellular matrix components during differentiation of adipocytes in long-term culture. In Vitro Cell. Dev. Biol. Anim. 36: 38–44.
Patrick CW (2000) Adipose tissue engineering: the future of breast and soft tissue reconstruction following tumor resection. Semin. Surg. Oncol. 19: 302–311.
Song H, O'Connor KC, Papadopoulos KD, Jansen DA (2002) Differentiation kinetics of in vitro 3T3-L1 preadipocyte cultures. Tissue Eng. 8: 1071–1081.
Spiegelman BM, Ginty CA (1983) Fibronectin modulation of cell shape and lipogenic gene expression in 3T3 adipocytes. Cell 35: 657–666.
Viravaidya K, Shuler ML (2001) The effect of various substrates on cell attachment and differentiation of 3T3-F442A preadipocytes. Biotechnol. Bioeng. 78: 454–458.
Voet D, Voet JG, Pratt CW (1999) Fundamentals of Biochemistry. New York: John Wiley & Sons, pp. 88–89.
von Heimburg D, Zachariah S, Heschel I, Kühling H, Schoof H, Hafemann B, Pallua N (2001) Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo. Biomaterials 22: 429–438.
Zuk PA, Zhu M, Mizuno H, Haung J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7: 211–228.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
O'Connor, K.C., Song, H., Rosenzweig, N. et al. Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose. Biotechnology Letters 25, 1967–1972 (2003). https://doi.org/10.1023/B:BILE.0000004386.08923.ab
Issue Date:
DOI: https://doi.org/10.1023/B:BILE.0000004386.08923.ab