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Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1773))

Abstract

Surgically discarded adipose tissue is not only an abundant source of multipotent adipose-derived stem/stromal cells (ASCs) but can also be decellularized to obtain a biomimetic microenvironment for tissue engineering applications. The decellularization methods involve processing excised fat through a series of chemical, mechanical, and enzymatic treatment stages designed to extract cells, cellular components, and lipid from the tissues. This process yields a complex 3D bioscaffold enriched in collagens that mimics the biochemical and biomechanical properties of the native extracellular matrix (ECM). For ASC culture and delivery, decellularized adipose tissue (DAT) provides a cell-supportive platform that is conducive to adipogenesis. While DAT can be applied in its intact form as an off-the-shelf adipogenic matrix, it can also be used as an ECM source for the fabrication of an array of other scaffold formats including adipose ECM-derived microcarriers and porous foams. In this chapter, we describe the methods developed in our lab to decellularize human adipose tissue and to further process it into a variety of scaffolding materials for a range of applications in soft tissue regeneration, wound healing, and cell culture.

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Author information

Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON, Canada

    Pascal Morissette Martin, Cody Brown & Lauren E. Flynn

  2. Department of Chemical and Biochemical Engineering, Thompson Engineering Building, The University of Western Ontario, London, ON, Canada

    Arthi Shridhar & Lauren E. Flynn

  3. Department of Chemical Engineering, Queen’s University, Kingston, ON, Canada

    Claire Yu & Lauren E. Flynn

Authors
  1. Pascal Morissette Martin
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  2. Arthi Shridhar
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  3. Claire Yu
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  4. Cody Brown
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  5. Lauren E. Flynn
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Corresponding author

Correspondence to Lauren E. Flynn .

Editor information

Editors and Affiliations

  1. Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA

    Bruce A. Bunnell  & Jeffrey M. Gimble  & 

  2. LaCell LLC, New Orleans, LA, USA

    Jeffrey M. Gimble

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Morissette Martin, P., Shridhar, A., Yu, C., Brown, C., Flynn, L.E. (2018). Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7799-4_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7797-0

  • Online ISBN: 978-1-4939-7799-4

  • eBook Packages: Springer Protocols

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