Skip to main content
SpringerLink
Log in

Immunohistochemical Analysis of Insulin-like Growth Factor–Binding Proteins -1, -2, and -3 in Implantation Sites of the Mouse

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor–binding proteins (IGFBPs) -1, -2, and -3 in implantation sites of the mouse.

Methods: Six-week-old B 6 D 2 F 1 female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin-embedded tissue was used for avidin–biotin immunocytochemical localization of IGFBPs utilizing standard methodology.

Results: Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5–10.5 days post coitum, there was moderate–dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua.

Conclusions: Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Rosenfield RG, Lamson G, Pham H, Oh YM, Conover C, DeLeon D, Donovan SM, Ocrant I, Giudice LC: Insulin-like growth factor binding proteins. Rec Progr Horm Res 1991;16:99–159

    Google Scholar 

  2. Kelley KM, Oh Y, Gargosky SE, Gucev A, Matsumoto T, Hwa V, Ng L, Simpson DM, Rosenfeld RG: Insulin-like growth factor-binding proteins (IGFBPs) and their regulatory dynamics. Int J Biochem Cell Biol 1996;28:619–637

    Google Scholar 

  3. Le Roith D: Insulin-like growth factor receptors and binding proteins. Bailliere's Clin Endocrinol Metab 1996;10:49–73

    Google Scholar 

  4. Bell SC, Jackson JA, Ashmore J, Zhu HH, Tseng L: Regulation of insulin-like growth factor-binding protein-1 synthesis and secretion by progestin and relaxin in long term cultures of human endometrial stromal cells. J Clin Endocrinol Metab 1991;72:1014–1024

    Google Scholar 

  5. Giudice LC, Milkowski DA, Fielder PJ, Irwin JC: Characterization of the steroid-dependence of insulin-like growth factor-binding protein-2 synthesis and mRNA expression in cultured human endometrial stromal cells. Hum Reprod 1991;6:632–640

    Google Scholar 

  6. Tseng L, Gao J-G, Chen R, Zhu HH, Mazella J, Powell DR: Effect of progestin, antiprogestin, and relaxin on the accumulation of prolactin and insulin-like growth factor-binding protein-1 messenger ribonucleic acid in human endometrial stromal cells. Biol Reprod 1992;47:441–450

    Google Scholar 

  7. Giudice LC, Dsupin BA, Irwin JC: Steroid and peptide regulation of insulin-like growth factor-binding proteins secreted by human endometrial stromal cells is dependent on stromal differentiation. J Clin Endocrinol Metab 1992;75:1235–1241

    Google Scholar 

  8. Liu H-C, He Z-Y, Mele C, Damario M, Davis O, Rosenwaks Z: Hormonal regulation of expression of messenger RNA encoding insulin-like growth factor binding proteins in human endometrial stromal cells cultured in vitro. Mol Hum Reprod 1997;3:21–26

    Google Scholar 

  9. Liu H-C, Mele C, Catz D, Noyes N, Rosenwaks Z: Production of insulin-like growth factor binding proteins (IGFBPs) by human endometrial stromal cells is stimulated by the presence of embryos. J Assist Reprod Genet 1995;12:78–87

    Google Scholar 

  10. Kapur S, Tamada H, Dey SK, Andrews GK: Expression of insulin-like growth factor-1 (IGF-1) and its receptor in peri-implantation mouse uterus, and cell-specific regulation of IGF-1 gene expression by estradiol and progesterone. Biol Reprod 1992;46:208–219

    Google Scholar 

  11. Rappolee DA, Sturm KS, Behrendtsen O, Schultz GA, Pedersen RA, Werb Z: Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos. Genes Dev 1992;6:939–952

    Google Scholar 

  12. Heyner S, Shah N, Smith RM, Watson AJ, Schultz GA: The role of growth factors in embryo production. Theriogenology 1993;39:151–161

    Google Scholar 

  13. Harvey MB, Kaye PL: Insulin-like growth factor-I stimulates growth of mouse preimplantation embryos in vitro. Mol Reprod Dev 1992;31:195–199

    Google Scholar 

  14. DeChiara TM, Efstratiadis A, Robertson EJ: A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting. Nature 1990;345:78–80

    Google Scholar 

  15. Kanai-Azuma M, Kanai Y, Kurohmaru M, Sakai S, Hayashi Y: Insulin-like growth factor (IGF)-I stimulates proliferation and migration of mouse ectoplacental cone cells, while IGF-II transforms them into trophoblastic giant cells in vitro. Biol Reprod 1993;48:252–261

    Google Scholar 

  16. Han VKM, Bassett N, Walton J, Challis JRG: The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: Evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab 1996;81:2680–2693

    Google Scholar 

  17. Hahnel A, Schultz GA: Insulin-like growth factor binding proteins are transcribed by preimplantation mouse embryos. Endocrinology 1994;134:1956–1959

    Google Scholar 

  18. Markoff E, Henemyre C, Fellows J, Pennington E, Zeitler PS, Cedars MI: Localization of insulin-like growth factor binding protein-4 expression in the mouse uterus during the peri-implantation period. Biol Reprod 1995;53:1103–1109

    Google Scholar 

  19. Sadek S, Unterman TG, Bell SC: Epithelial localization of insulin-like growth factor binding protein 1 in the uterus of the rat during pregnancy, deciduoma-bearing pseudopregnancy and hormone treatment. J Reprod Fertil 1994;101:67–75

    Google Scholar 

  20. Croze L, Kennedy TG, Schroedter IC, Friesen HG, Murphy LJ: Expression of insulin-like growth factor-I and insulin-like growth factor binding protein-1 in the rat uterus during decidualization. Endocrinology 1996;71:1995–2000

    Google Scholar 

  21. Boomsma RA, Mavrogianis PA, Fazleabas AT, Jaffe RC, Verhage HG: Detection of insulin-like growth factor binding protein-1 in cat implantation sites. Biol Reprod 1994;51:392–399

    Google Scholar 

  22. Tarantino S, Verhage HG, Fazleabas AT: Regulation of insulin-like growth factor-binding proteins in the baboon (Papio anubis) uterus during early pregnancy. Endocrinology 1992;130:2354–2362

    Google Scholar 

  23. Irwin JC, Dsupin BA, Giudice LC: Modulation of cytotrophoblast interaction with fibronectin and endometrial stromal cell cultures by insulin-like growth factor binding protein-1 (IGFBP-1). Presented at the Society for the Study of Reproduction, Sacramento, CA, July 1995

  24. Irving JA, Lala PK: Functional role of cell surface integrins on human trophoblast cell migration: regulation by TGF-β, IGF-II, and IGFBP-1. Exp Cell Res 1995;217:419–429

    Google Scholar 

  25. Lee PDK, Conover CA, Powell DR: Regulation and function of insulin-like growth factor binding protein-1. Proc Soc Exp Biol Med 1993;204:4–29

    Google Scholar 

  26. Sara VR, Hall K: Insulin-like growth factors and their binding proteins. Physiol Rev 1990;70:591–614

    Google Scholar 

  27. Blum WF, Jenne EW, Reppin T, Kietzmann K, Ranke MB, Bierich JR: Insulin-like growth factor I (IGF-I)-binding protein complex is a better mitogen than free IGF-I. Endocrinology 1989;125:766–772

    Google Scholar 

  28. Conover CA: Potentiation of insulin-like growth factor (IGF) action by IGF-binding protein-3: Studies of underlying mechanism. Endocrinology 1992;130:3191–3199

    Google Scholar 

  29. Koistinen R, Angervo M, Leinonen P, Seppala M: Phosphorylation of insulin-like growth factor-binding protein-1 from different sources. Growth Reg 1993;3:34–37

    Google Scholar 

  30. Jones JI, D-Ercole AJ, Camacho-Hubner C, Clemmons DR: Phosphorylation of insulin-like growth factor (IGF)-binding protein 1 in cell culture and in vivo: Effects on affinity for IGF-1. Proc Natl Acad Sci USA 1991;88:7481–7485

    Google Scholar 

Download references

Author information

Author notes

  1. Marcelo G. Horenstein

    Present address: Department of Pathology, Duke University Medical Center, Durham, North Carolina

Authors and Affiliations

  1. Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, Cornell University Medical College, New York, New York

    Mark A. Damario, Hung-Ching Liu, Carol Ann Mele & Zev Rosenwaks

  2. Department of Pathology, Cornell University Medical College, New York, New York

    Marcelo G. Horenstein

Authors
  1. Mark A. Damario
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hung-Ching Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carol Ann Mele
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marcelo G. Horenstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zev Rosenwaks
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Damario, M.A., Liu, HC., Mele, C.A. et al. Immunohistochemical Analysis of Insulin-like Growth Factor–Binding Proteins -1, -2, and -3 in Implantation Sites of the Mouse. J Assist Reprod Genet 15, 513–520 (1998). https://doi.org/10.1023/A:1022538621928

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022538621928

Search

Navigation