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Genetic relationships and diversity among Tibetan wheat, common wheat and European spelt wheat revealed by RAPD markers

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Abstract

An endemic hexaploid wheat found in Tibet, China was taxonomically classified as a subspecies in common wheat, i.e. Triticum aestivum ssp. tibetanum. Seven accessions of the Tibetan wheat, 22 cultivars of common wheat and 17 lines of spelt wheat were used for RAPD analysis to study the genetic relationships of the Tibetan wheat with common wheat and spelt wheat, and to assess the genetic diversity (GD) among and within the taxa. RAPD polymorphism was found to be much higher within spelt wheat and the Tibetan wheat than within common wheat. The GD value between the Tibetan wheat and common wheat is lower than that between the Tibetan wheat and spelt wheat. The result of cluster analysis showed that the 46 genotypes were distinctly classified into two groups. Group 1 included all European spelt wheat lines, while group 2 includes all Chinese common wheat and the Tibetan wheat accessions. However, the Tibetan wheat was substantially differentiated from Chinese common wheat at a lower hierarchy. Our results support an earlier classification of the Tibetan wheat as a subspecies in common wheat. European spelt wheat and the Tibetan wheat showed much higher genetic diversity than Chinese common wheat, which could be used to diversify the genetic basis for common wheat breeding.

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References

  • Abo-elwafa, A., K. Murai & T. Shimada, 1995. Intra-and Inter-species variation in Lens revealed by RAPD markers. Theor Appl Genet 90: 335–340.

    Article  CAS  Google Scholar 

  • Barbosa-Neto, J.F., M.E. Sorrells & G. Cisar, 1996. Prediction of heterosis in wheat using coefficient of parentage and RFLP-based estimates of genetic relationships. Genome 39: 1142–1149.

    CAS  PubMed  Google Scholar 

  • Chen, H.B., J.M. Martin, M. Lavin & L.E. Talbert, 1994. Genetic diversity in hard red spring wheat based on sequence-targeted-site PCR markers. Crop Sci 34: 1628–1632.

    Article  CAS  Google Scholar 

  • Chen, P.D., D.J. Liu, G.Z. Pei, L.L. Qi & L. Huang, 1991. The chromosome constitution of three endemic hexaploid wheats in western China. Proc 7th International Wheat Genetics Symposium, pp. 75–80.

  • Cox, T.S., J.P. Murphy & D.M. Rodgers, 1986. Changes in genetic diversity in the red winter wheat regions in the United States. Proc Natl Acad Sci USA 83: 5583–5586.

    Article  PubMed  CAS  Google Scholar 

  • Gonzalez, J.M. & E. Ferrer, 1993. Random amplified polymorphic DNA analysis in Hordeum species. Genome 36: 1029–1031.

    CAS  PubMed  Google Scholar 

  • Graham, J. & R.J. McNicol, 1995. An examination of the ability of RAPD markers to determine the relationships within and between Rubus species. Theor Appl Genet 90: 1128–1132.

    Article  CAS  Google Scholar 

  • Heun, M., J.P. Murphy & T.D. Phillips, 1994. A comparison of RAPD and isozyme analysis for determining the genetic relationships among Avena sterilis L. accessions. Theor Appl Genet 87: 689–696.

    Article  CAS  Google Scholar 

  • Huang Henglu, Ma Dequan, Zhou Ronghua, Xie Fuchang, Gu Maozhi & Wu Shubao, 1987. The kinds, distributions and preliminary studies of Xizang fragile spike wheat. Proc Exploration on Crop Germplasms in Tibet, pp. 33–39. Publishing House of Chinese Agricultural Sciences.

  • Joshi, C.P. & H.T. Nguyen, 1993. Application of the random amplified polymorphic DNA technique for the detection of polymorphisms among wild and cultivated tetraploid wheats. Genome 36: 602–609.

    PubMed  CAS  Google Scholar 

  • Link, W., C. Dixkens, M. Singh, M. Schwall & A.E. Melchinger, 1995. Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD. Theor Appl Genet 90: 27–32.

    Article  CAS  Google Scholar 

  • Liu, C.J., 1996. Genetic diversity and relationships among Lablab purpureum genotypes evaluated using RAPD as markers. Euphytica 90: 115–119.

    Article  Google Scholar 

  • Liu, Y.G. & K. Tsunewaki, 1990. Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic DNA library construction and RFLP analysis in common wheat. Jap J Genet 65: 367–380.

    Article  CAS  Google Scholar 

  • Martin, J.M., L.E. Talbert, S.P. Lanning & N.K. Blake, 1995. Hybrid performance in wheat as related to parental diversity. Crop Sci 35: 104–108.

    Article  Google Scholar 

  • M'Ribu, H.K. & K.W. Hilu, 1994. Detection of intraspecific and intraspecific variation in Panicum millets through random amplified polymorphic DNA. Theor Appl Genet 88: 412–416.

    Article  Google Scholar 

  • Nei, M. & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76: 5269–5273.

    Article  PubMed  CAS  Google Scholar 

  • Plaschke, J., M.W. Ganal & M.S. Roder, 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91: 1001–1007.

    Article  CAS  Google Scholar 

  • Shao Qiquan, Li Changsen & Basang Ciren, 1980. Semi-wild wheat from Xizang (Tibet). Acta Genetica Sinica 7(2): 149–156.

    Google Scholar 

  • Siedler, H., M.M. Messmer, G.M. Schachermayr, H. Winzeler & B. Kellerl, 1994. Genetic diversity in European wheat and spelt breeding materials based on RFLP data. Theor Appl Genet 88: 994–1003.

    Article  Google Scholar 

  • Sun, Qixin, T.C. Huang, Z.F. Ni & J.D. Procunier, 1996. Study on wheat heterotic group. I. Genetic diversity revealed by random amplified polymorphic DNA (RAPD) in elite wheat cultivars. J Agric Biotechnol 4(2): 103–110.

    Google Scholar 

  • Vierling, R.A. & H.T. Nguyen, 1992. Use of RAPD markers to determine the genetic relationships of diploid wheat genotypes. Theor Appl Genet 84: 835–838.

    Article  CAS  Google Scholar 

  • Zhang, Qifa, M.A. Saghai Maroof, T.Y. Lu & B.Z. Shen, 1992. Genetic diversity and differentiation of indica and japonica rice detected by RFLP analysis. Theor Appl Genet 83: 495–499.

    Google Scholar 

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Sun, Q., Ni, Z., Liu, Z. et al. Genetic relationships and diversity among Tibetan wheat, common wheat and European spelt wheat revealed by RAPD markers. Euphytica 99, 205–211 (1998). https://doi.org/10.1023/A:1018316129246

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  • DOI: https://doi.org/10.1023/A:1018316129246

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