Abstract
A review is presented of the recent developments in the metabolism andfunction of polyamines in plants. Polyamines appear to be involved in a widerange of plant processes so their exact role is not completely understood. Inthis review, the metabolic pathways involved in polyamine biosynthesis anddegradation are explained, along with the transport and conjugation of thesecompounds. The methodologies involved in the analysis of polyamine functionusing metabolic inhibitors and genetic and molecular approaches are described.The occurrence and distribution of polyamine-derived alkaloids are also dealtwith. The direction of future research in the study of plant polyamines isindicated.
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Altabella T., Taylor M.A. and Tiburcio A.F. 1997. Recent advances in polyamine research. Trends Plant Sci. 2: 124–130.
Altamura M.M. 1993. Cytological events induced by inhibition of polyamine biosynthesis in thin cell layers of tobacco. Protoplasma. 175: 9–16.
Angelini R., Federico R. and D'Ovidi R. 1996. Spatial distribution and temporal accumulation of mRNA encoding diamine oxidase during lentil (Lens culinaris Medicus) seeling development. Plant Sci. 119: 103–113.
Antognoni F., Pistocchi R., Casali P. and Bagni B. 1995. Does calcium regulate polyamine uptake in carrot protoplasts?. Plant Physiol. Biochem. 33: 701–702.
Apelbaum A., Camellakis Z.N., Applewhite P.B., Kaur-Sawhney R. and Galston A.W. 1988. Binding of spermidine to a unique protein in thin-layer tobacco tissue culture. Plant Physiol. 88: 996–998.
Aribaud M. and Martin-Tangu J. 1994. Polyamine metabolism in normal and sterile chrysanthemum plants. Phytochemistry. 37: 927–932.
Aribaud M., Carré M. and Martin-Tanguy J. 1994. Polyamine metabolism and in vitro cell multiplication and differentiation of chrysanthemum leaf explants. Plant Growth Regul. 15: 143–155.
Bagni N. and Pistocchi R. 1991. Uptake and transport of polyamines and inhibitors of polyamine metabolism in plants. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in plants. CRC Press, Boca Raton, pp. 105–118.
Bajaj S. and Rajam M.V. 1995. Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell. Rep. 14: 717–720.
Baldwin I.T. 1988. Damage-induced alkaloids in tobacco: potbound plants are not inducible. J. Chem. Ecol. 14: 1113–1115.
Bardocz S. 1993. The role of dietary polyamines. Eur. J. Clin. Nutr. 47: 683–690.
Basu H.S., Schwietert H.C.A., Feuerstein B.G. and Marton L.J. 1990. Effect of variation in the structure of spermine on the association with DNA and the induction of DNA conformational changes. Biochem. J. 269: 329–334.
Beigbeder A.R. 1995. Influence of polyamine inhibitors on light independent and light dependent chlorophyll biosynthesis and on the photosyntetic rate. J. Photochem. Photobiol. 28: 235–242.
Besford R.T., Richardson C.M., Campos J.L. and Tiburcio A.F. 1993. Effect of polyamines on stabilization of molecular complexes of thylakoid membranes of osmotically stressed oat leaves. Planta. 189: 201–206.
Birecka H., Birecki M. and Frohlich M.W. 1987. Evidence for arginine as the endogenous precursor of necines in Heliotropiwn. 84: 42–46.
Bonneau L., Carré M. and Martin-Tanguy J. 1994. Polyamines and related enzymes in rice differing in germination potential. 15: 75–82.
Borrell A., Culianez-Macia F.A., Altabella T., Besford R.T., Flores D. and Tiburcio A.F. 1995. Arginine decarboxylase is localized in chloroplasts. Plant Physiol. 109: 771–776.
Borrell A., Besford R.T., Altabella T., Masgrau C. and Tiburcio A.F. 1996. Regulation of arginine decarboxylase by spermine in osmotically-stressed oat leaves. Physiol. Plant. 98: 105–110.
Burtin D., Martin-Tanguy J., Paynot M., Carré M. and Rossin M. 1990. Polyamines, hydroxycinnamoylputrescines, and root formation in leaf explants of tobacco cultivated in vitro. Effects of the suicide inhibitors of putrescine synthesis. Plant Physiol. 93: 1398–1404.
Burtin D., Martin-Tanguy J. and Tepfer D. 1991. α-DL-difluoromethylornithine, a specific irreversible inhibitor of putrescine synthesis, induces a phenotype in tobacco similar to that ascribed to the root-inducing, left-hand transferred DNA of Agrobacterium rhizo genes. Plant Physiol. 95: 461–468.
Campos J.L., Figueras X., Boronat A., Pinol M.T. and Tiburcio A.F. 1991. Changes in polyamine content of Arabidopsis thaliana after UV-C irradiation. In: Galston G.W. and Tiburcio A.F. (eds), Lecture Course on Polyamines as Regulators of Plant Development. Fundation Juan March, pp. 78–80.
Del Duca S., Tidu V., Bassi R., Esposito C. and Serafini-Fracassini D. 1994. Identification of Chi a/b proteins as substrates of transglutaminase activity in isolated chloroplasts of Helianthus suberosus. L. Planta. 193: 283–289.
Del Duca S., Beninati S. and Serafini-Fracassini D. 1995. Polyamines in chloroplast:Identification of their glutamyl and acetyl derivatives. Biochem J. 305: 233–237.
De Scenzo R.A. and Minocha S.C. 1993. Modulation of cellular polyamines in tobacco by transfer and expression of mouse ornithine decarboxylase. Plant Mol. Biol. 22: 113–127.
D'Oraci D. and Bagni N. 1987. In vitro interactions between polyamines and pectic substances. Biochem. Biophys. Res. Commun. 148: 1159–1163.
Ecker J.R. 1994. The ethylene signal transduction pathways in plants. Science. 268: 327–338.
Egea-Cortines M. and Mizrahi Y. 1991. Polyamines in cell division, fruit set and development, and seed germination. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in plants. CRC Press, Boca Raton, pp. 143–158.
El Ghachtoul N., Martin-Tangu J., Paynot M. and Gianinazz S. 1996. First report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic treatment. FEBS Lett. 385: 189–192.
Evans P.T. and Malmberg R.L. 1989. Do polyamines have roles in plant development?. Ann. Rev. Plant Physiol. Plant Mol. Biol. 40: 235–269.
Fecker L.F., Hiilebrandt S., Rügenhagen C., Herminghau S., Landsmann J. and Berlin J. 1992. Metabolic effects of a bacterial lysine decarboxylase gene expressed in a hairy root culture of Nicotiana glauca. Biotechnol. Lett. 14: 1035–1040.
Federico R. and Angelini R. 1991. Polyamine catabolism. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in plants. CRC Press, Boca Raton, pp. 41–56.
Feth F., Wagner R. and Wagner K.G. 1986. Regulation in tobacco callus of enzyme activities of the nicotine pathway. Planta. 108: 402–410.
Flores H.E. and Filner P. 1985. Polyamine catabolism in higher plants: characterization of pyrroline dehydrogenase. Plant Growth Regul. 3: 277–291.
Flores H.E., Protacio C.M. and Signs M.W. 1989. Primary and secondary metabolism of polyamines in plants. Recent. Adv. Phytochem. 23: 329–393.
Flores H.E. and Martin-Tanguy J. 1991. Polyamines and Plant Secondary Metabolites. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in Plants. CRC Press, Boca Raton, pp. 57–72.
Folk J.E. 1980. Transglutaminases. Annu. Rev. Biochem. 49: 517–531.
Fritze K., Czaja I. and Walden R. 1995. T-DNA tagging of genes influencing polyamine metabolism: isolation of mutant plant lines and rescue of DNA promoting growth in the presence of a polyamine biosynthetic inhibitor. Plant. 7: 261–271.
Galston A.W. and Kaur-Sawhney R.K. 1990. Polyamines in plant physiology. Plant Physiol. 94: 406–410.
Galston A.W. and Flores H.E. 1991. Polyamines in plant morphogenesis. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in plants. CRC Press, Boca Raton, pp. 175–183.
Galston A.W. and Kaur-Sawhney R. 1995. Polyamines as endogenous growth regulators. In: Davies P.J. (ed.), Plant Hormones. Physiology, Biochemistry and Molecular Biologiy. 2nd edn. Kluwer Academic Publishers, Dordrecht, pp. 158–178.
Geny L., Broquedis M., Martin-Tanguy J. and Bouard J. 1997. Free, conjugated and wall-bound polyaniines in various parts of fruiting cuttings of Vitis vinifera at different stages of development. Am. J. Enol. Vitic. 48: 80–84.
Gilad G.M., Gilad V.H. and Rabey J.M. 1996. Arginine and ornithine decarboxylation in rodent brain-coincidental changes during development and after ischemia. Neurosci. Lett. 216: 33–36.
Guggisberg A. and Hesse M. 1983. Putrescine, spermidine, spermine and related polyamine alkaloids. In: Brossi A. (ed.), The Alkaloids, Chemistry and Pharmacology. vol. XXII Academic Press, New York, p. 85.
Hachiya A., Yamamoto Y. and Matsumoto H. 1996. Inhibitory effect of phenylpropanoids on aluminium toxicity in cultured tobacco cells. Plant Cell Physiol. 37: 53.
Hartman T., Sander H., Adolph M. and Toppel G. 1988. Metabolic links between the biosynthesis of pyrrolizidine alkaloids and polyamines in root cultures of Senecio vulgaris. Planta. 175: 82–90.
Havelange A., Lejeune P., Bernier A., Kaur-Sawhney R. and Galston A.W. 1996. Putrescine export from leaves in relation to floral transition in Sinapis alba. Physiol. Plant. 96: 59–65.
Hayashi S. and Murakami Y. 1995. Rapid and regulated degradation of ornithine decarboxylase. Biochem. J. 306: 221–230.
Heby O. and Persson L. 1990. Molecular genetics of polyamine synthesis in eukaryotic cells. Trends. Biochem. Sci. 15: 153–158.
Hibi N., Fujita T., Hatamo M., Hashimoto T. and Gamada Y. 1992. Putrescine N-methyl transferase in cultured roots of Hyoscyanius albus. n-Butylamine as a potent inhibitor of the transferase both in vitro and in vivo. Plant Physiol. 100: 826–835.
Hibi N., Higashiguchi S., Hashimoto T. and Yamada Y. 1994. Gene expression in tobacco low-nicotine mutants. Plant Cell. 6: 723–735.
Jansen N.A.K., Gaba V. and Greenberg M. 1998. Higher plants and UV-B radiation balancing damage, repair and acclimation. Trends. Plant Sci. 3: 119–159.
Kallio A., McCann P. and Bey P. 1981. DL_α(Difluoromethyl)arginine: a potent enzyme activated irreversible inhibitor of bacterial arginine decarboxylase. Biochemistry. 20: 3163–3166.
Kumar A., Taylor M.A., Mad Arif S.A. and Davies H.V. 1996. Potato plants expressing antisense and sense S-adenosylmethionine decarboxylase (SAMDC) transgenes show altered levels of polyamines and ethylene: Antisense plants display abnormal phenotypes. Plant J. 9: 147–158.
Kupchan S.M., Davies A.P., Barboutis S.J., Schnoes H.K. and Burlingame A.L. 1969. Tumor inhibitors. XLIII. Solapalmitine and solapalmitenine, two novel alkaloid tumor inhibitors from Solanum tripartitum. J. Org. Chem. 34: 3888–3898.
Langebartels C., Kerner K.J., Leonardi S., Schraudner M., Trost M., Heller W. et al. 1991. Biochemical plant response to ozone. I. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiol. 91: 882–887.
Li G., Regunathan S., Barrow C.J., Eshraghi J., Cooper R. and Reiss D.J. 1994. Agmatine – An endogenous clonidine-displacing substance in the brain. Science. 263: 966–969.
Mad Arif S.A., Taylor M.A., George L.A., Buder A.R., Burch L.R., Davies H.U. et al. 1994. Characterisation of the S-adenosylmethionine decarboxylase (SAM DC) gene of potato. Plant Mol. Biol. 26: 327–338.
Malmberg R.L. and Cellino M.L. 1994. Arginine decarboxylase of oat is activated by enzymatic cleavage into two polypeptides. J. Biol. Chem. 28: 2703–2706.
Martin-Tanguy J. 1985. The occurrence and possible functions of hydroxycinnamoyl acid amides in plant. Plant Growth Regul. 3: 381–399.
Martin-Tanguy J. 1987. Hydroxycinnamic acid amides, hypersensitivity, flowering and sexual organogenesis in plants. In: Von Wettstein D. and Chua D.N. (eds), Plant Molecular Biology. Plenum Publishing Corporation, New York, pp. 253–263.
Martin-Tanguy J., Tepfer D., Paynot M., Burtin D., Heisler I. and Martin C. 1990. Inverse relationship between polyamine levels and the degree of phenotypic alteration induced by the root-inducing left-hand transferre DNA from Agrobacterium rhizogenes. Plant Physiol. 92: 912–918.
Martin-Tanguy J., Corbineau F., Burtin D., Ben Hayyim G. and Tepfer D 1993. Genetic transformation with a derivative of rol C from Agrobacterium rhizogenes or treatment with α-aminoisobutyric acid reduces ethylene production and the accumulation of water-insoluble polyaminehydroxycinnamic acid conjugates in tobacco flowers. Plant Sci. 93: 63–76.
Martin-Tanguy J., Sun L.Y., Burtin D., Vernoy R., Rossin N. and Tepfer D. 1996. Attenuation of the phenotypes caused by rootinducing, left-hand transferred DNA and its rol A gene. Correlations with changes in polyamine metabolism and DNA methylation. Plant Physiol. 111: 259–267.
Martin-Tanguy J. 1997. Conjugated polyamines and reproductive development: biochemical, molecular and physiological approaches. Physiol. Plant. 100: 675–688.
Masgrau C., Mtabella T., Farras R., Flores P., Thompson A.J., Besford R.T. et al. 1997. Inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. Plant J. 11: 465–473.
Mehta H.S., Saftner R.A., Mehta R.A. and Davies P.J. 1994. Identification of posttranscriptionally modified 18-kilodalton protein from rice as eukaryotic translocation initiation factor 5A. Plant Physiol. 106: 1413–1419.
Metcalf B.W., Bey P., Danzin C., Jung M.J., Casara P. and Vevert J.P. 1978. Catalytic irreversible inhibition of mammalian ornithine decarboxylase by substrat and product analogues. J. Am. Chem. Soc. 100: 2251–2253.
Meurer-Grimes B., Berlin J. and Strack D. 1989. Hydroxycinnamoyl-CoA: putrescine hydroxycinnamoyl transferase in tobacco cell cultures with high and low levels of caffeoylputrescine. Plant Physiol. 89: 488–492.
Michael A.J., Furze J.M., Rhodes J.C. and Burtin D. 1996. Molecular cloning and functional identification of a plan ornithine decarboxylase cDNA. Biochem. J. 314: 241–248.
Minocha S.C. and Minocha R.C. 1996. Role of polyamines in somatic embryogenesis. In: Bajaj Y.P.S. (ed.), Biotechnology in Agriculture and Forestry: Somatic Embryogenesis and Synthetic Seeds. vol. 30 Springer-Verlag, New York, pp. 53–70.
Mueller S. 1996. QTL analysis of polyamines in potato leaves. In: Struick P.C. (ed.), Abstracts of 13th Triennial Conference of the European Association for Potato Research. Veldhoven, pp. 73–74.
Negrel J. and Martin C. 1984. The biosynthesis of feruloyl tyramine in Nicotiana tabacum. Phytochemistry. 23: 2797–2801.
Negrel J. and Lherminier J. 1987. Peroxidase-mediated integration of tyramine into xylem cell walls of tobacco leaves. Planta. 72: 494–501.
Negrel J. 1989. The biosynthesis of cinnamoylputrescines in callus tissue cultures of Nicotiana tabacum. Phytochemistry. 28: 477–481.
Pelosi L.A., Rother A. and Edwards J.M. 1986. Lysine decarboxylase activity and alkaloid production in Heimia saliczfolia cultures. Phytochemistry. 25: 2319–2915.
Perez-Amador M.A., Carbonell J. and Granell A. 1995. Expression of arginine decarboxylase is induced during early fruit development and in young tissues of Pisum sativum (L.)-. Plant Mol. Biol. 28: 997–1009.
Philipps G.C. and Kuehn G.D. 1991. Uncommon polyamines in plants and other organisms. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in Plants. CRC Press, Boca Raton, pp. 121–133.
Pistoochi R., Antognoni F., Bagni N. and Zannoni D. 1990. Spermidine uptake by mitochondria of Hehanthus tuberosus. Plant Physiol. 92: 690–695.
Pistocchi R. and Bagni N. 1990. Effect of calcium on spermine uptake in carrot cell cultures and protoplasts. J. Plant Physiol. 136: 728–733.
Pohjanpelto P. and Höltta E. 1996. Phosphorylation of Okazaki-like DNA fragments in mammalian cells and role of polyamines in the processing of this DNA. EMBO J. 15: 1193–1200.
Robins R.J., Parr A.J. and Walton N.J. 1991. Studies on the biosynthesis of tropane alkaloids in Datura stramornum L. transformed root cultures. 2. On the relative contributions of L-arginine and L-ornithine to the formation of the tropane ring. Planta. 183: 196.
Schoofs G., Teichman S., Hartmann T. and Wink M. 1983. Lysine decarboxylase activity and alkaloid production in Heimia salicifolia cultures. Phytochemistry. 25: 2315–2317.
Schröder G. and Schröder J. 1995. CDNAs from Catharanthus roseus heterologous expression, identification of the proenzymeprocessing site, evidence for the presence of both subunits in the active enzyme, and a conserved region in the 5′-mRNA leader. Eur. J. Biochem. 228: 74–78.
Schuber F., Hong K., Duözgünes N. and Papahadjopoulos D. 1983. Polyamines as regulators of membrane fusion: aggregation and fusion of liposomes. Biochemistry. 22: 6134–6140.
Serafini-Fracassini D., Del Duca S. and Beninati S. 1995. Plant transglutaminases. Phytochemistry. 40: 355–365.
Slocum R.D. 1991. Tissue and subcellular localisation of polyamines and enzymes of polyamine metabolism. In: Slocum R.D. and Flores H.E. (eds), Biochemistry and Physiology of Polyamines in plants. CRC Press, Boca Raton, pp. 93–105.
Smith T.A., Negrel J. and Bird C.R. 1983. The cinnamic acid amides of the di-and polyamines. In: Bachrach U., Kayc A. and Chayen R. (eds), Advances in Polyamine Research. Raven Press, New York, pp. 347–370.
Tabor C.W. and Tabor H. 1984. Polyamines. Annu. Rev. Biochem. 53: 749–790.
Tarenghi E. and Martin-Tanguy J. 1995. Polyamines, floral induction and floral development of strawberry (Fragaria ananassa Duch.). Plant Growth Regul. 17: 157–165.
Tassoni A., Antognoni F. and Bagni N. 1996. Polyamine binding to plasma membrane vesicles from zucchini hypocotyls. Plant Physiol. 110: 817–824.
Tepfer D. 1984. Transformation of several species of higher plants by Agrobacterium rhizo genes: Sexual transmission of the transformed genotype and phenotype. Cell. 47: 959–967.
Tiburcio A.F., Kaur-Sawhney R., Ingersoll R.B. and Oalston A.W. 1985. Correlation between polyamines and pyrrolidine alkaloids in developing tobacco callus. Plant Physiol. 78: 323–326.
Tiburcio A.F., Kaur-Sawhney R. and Galston A.W. 1987. Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures. Plant Cell Tissue Organ Culture. 9: 111–120.
Tiburcio A.F., Kaur-Sawhney R. and Galston A.W. 1990. Polyamine metabolism. In: Miflin B.J. and Lea P.J. (eds), The Biochemistry of Plants, Intermediary Nitrogen Fixation. Academic Press, New York, pp. 283–325.
Tiburcio A.F., Altabella T., Borrell A. and Masgraw C. 1997. Polyamine metabolism and its regulation. Physiol. Plant. 100: 664–674.
Tipping A.J. and McPherson M.J. 1995. Cloning and molecular analsyis of pea seedling copper amine oxidase. J. Biol. Chem. 270: 16939–16946.
Twardowski T., Puhkowska J. and Wiewiorowski M. 1982. Inhibitory effect of selected quinolizidine alkaloids and their derivatives and analogues on the Phe-tRNA binding to ribosomes. Bull. Acad. Pol. Sc. 29: 129–140.
Watson M. and Malmberg R.L. 1996. Regulation of Arabidopsis thaliana (L.) Heynh arginine decarboxylase by potassium defi-ciency stress. Plant Physiol. 111: 1077–1083.
Wink M. and Hartman T. 1982. Localization of the enzyme of quinolizidine alkaloid biosynthesis in leaf chloroplasts of Lupinus polyphyllus. Plant Physiol. 70: 74–77.
Wink M. 1985. Metabolism of quinolizidine alkaloids in plants and cell suspension cultures. Induction and degradation. In: Neumann K.H., Barz W. and Reinhand E. (eds), Primary and secondary metabolism of plant cell cultures. Springer-Verlag, Heidelberg, pp. 107–116.
Ye X.S., Avdiushko S.A. and Kuc J. 1994. Effect of polyamines on in vitro phosphorylation of soluble and plasma membrane proteins in tobacco, cucumber and Arabidopsis thaliana. Plant Sci. 97: 109–118.
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Martin-Tanguy, J. Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regulation 34, 135–148 (2001). https://doi.org/10.1023/A:1013343106574
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DOI: https://doi.org/10.1023/A:1013343106574