Abstract
The observations reported in this article demonstrate that lipoic acid strongly influences the activity of a purified preparation of choline acetyl transferase. The reduced form, dihydrolipoic acid, is a powerful activator of the enzyme while lipoic acid itself has an inhibitory effect and counteracts the stimulatory effect of dihydrolipoic acid. It is proposed that dihydrolipoic acid serves an essential function in the action of this enzyme and that the ratio of reduced to oxidized lipoic acid in the cell may play an important role in the regulation of the activity of the enzyme. The implications of these findings for cell function and acetyl choline formation are discussed.
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Bustamante J, Lodge JK, Marcocci L, Tritchhler HJ, Packer L, Rihn BH: α-Lipoic acid in liver metabolism and disease. Free Rad Biol Med 24: 1023-1039, 1998
Packer L, Tritschler HJ, Wessel K: Neuroprotection by the metabolic antioxidant α-lipoic acid. Free Rad Biol Med 22: 359-378, 1997
Haugaard N, Haugaard ES: Stimulation of glucose utilization by thioctic acid in rat diaphragm incubated in vitro. Biochim Biophys Acta 222: 583-586, 1970
Singh HPP, Bowman RH: Effect of DL-α-lipoic acid on the citrate concentration and fructophosphokinase activity by perfused hearts from normal and diabetic hearts. Biophys Biochem Res Commun 41: 555-561, 1970
Henriksen EJ, Jacob S: Chronic thioctic acid treatment increases insulin stimulated glucose transport activity in skeletal muscle of obese Zucker rats. Diabetes 43(suppl): 122A, 1994
Jacob S, Streeper RS, Fogt DL, Hokama JY, Tritschler HJ, Dietze GJ, Henriksen EJ: Metabolism in insulin-resistant rat skeletal muscle. Diabetes 45: 1024-1029, 1996
Henriksen EJ, Jacob S, Streeper RS, Fogt DL, Hokama JY, Tritchler HJ: Stimulation by α-lipoic acid of glucose transport activity in skeletal muscle of lean and obese Zucker rats. Life Sci 61: 805-812, 1997
Sachse G, Williams B: Efficacy of thioctic acid in the therapy of peripheral diabetic neuropathy. In: F.A. Gries, H.J. Freund, F. Rabe, H. Berger (eds). Aspects of Autonomic Neuropathy in Diabetes. Horm Metab Res 9: 105-108, 1980
Ziegler D, Hanafeld M, Ruhnau KJ, Meisner HP, Lobisch M, Schutte K, Gries FA: Treatment of symptomatic diabetic peripheral neuropathy with the antioxidant α-lipoic acid. Diabetologia 38: 1425-1433, 1996
Tucek S: The synthesis of acetyl choline. In: A. Lajtha (ed). Handbook of Neurochemistry, vol 4. Plenum Press, New York, 1983, 219-249
Fonnum F: A rapid radiochemical method for the determination of choline acetyltransferase. J Neurochem 24: 407-409, 1975
Patel MS, Roche TE, Harris RA: Alpha-Keto Acid Dehydrogenase Complexes. Molecular and Cell Biology Updates. Birkhauser Verlag, Basel, 1996
Haugaard N: Reflections on the role of the thiol group in biology. Ann NY Acad Sci 899: 148-158, 2000
Haugaard N, Lee NH, Kostrzewa R, Horn RS, Haugaard ES: The role of sulfhydryl groups in oxidative phosphorylation and ion transport by rat liver mitochondria. Biochim Biophys Acta 172: 198-204, 1969
Sen CK: Redox signalling and the emerging therapeutic potential of thiol antioxidants. Biochem Pharmacol 55: 1747-1758, 1998
Packer L, Roy S, Sen CK: α-Lipoic acid: A metabolic antioxidant and potential redox modulator of transcription. Adv Pharmacol 38: 79-101, 1997
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Haugaard, N., Levin, R.M. & Surname, F. Regulation of the activity of choline acetyl transferase by lipoic acid. Mol Cell Biochem 213, 61–63 (2000). https://doi.org/10.1023/A:1007156732662
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DOI: https://doi.org/10.1023/A:1007156732662