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The Role of Lysophospholipid Acyltransferases in the Golgi Complex

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The Golgi Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1496))

Abstract

Determining the abundance of phospholipids and neutral lipids in cellular membranes is paramount to understanding their biological functions. Many lipid-modifying enzymes have yet to be characterized due to limitations in substrate–product measurements and purification of membrane-bound enzymes. The method described here uses radiolabeled phospholipid substrates and cell-purified organelles to quantify phospholipid metabolism using thin-layer chromatography. This assay has the benefits of being specific and adaptable for numerous applications and systems.

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Acknowledgements

Thank you William J. Brown for critical reading of the manuscript and Edward Cluett, Dan Drecktrah, and Kimberly Chambers for their intellectual contributions.

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Authors and Affiliations

  1. Department of Biology, Villanova University, Mendel Hall Rm G63B, 800 Lancaster Avenue, Villanova, PA, 19085, USA

    John A. Schmidt

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  1. John A. Schmidt
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Correspondence to John A. Schmidt .

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Editors and Affiliations

  1. Dept of Molecular Biology & Genetics, Cornell University, Ithaca, New York, USA

    William J. Brown

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Schmidt, J.A. (2016). The Role of Lysophospholipid Acyltransferases in the Golgi Complex. In: Brown, W. (eds) The Golgi Complex. Methods in Molecular Biology, vol 1496. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6463-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6463-5_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6461-1

  • Online ISBN: 978-1-4939-6463-5

  • eBook Packages: Springer Protocols

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