Abstract
The gynogenic response of a range of onion genotypes to flower bud culture was compared using a two-step culture system. Embryogenic cultures and plantlets were produced from unpollinated ovules in whole flower bud explants 6 to 19 weeks after culture initiation. Preconditioning stock plants significantly influenced gynogenic embryogenesis. A ten-fold increase in embryogenesis was obtained when flower buds were cultured from stock plants maintained at 15 °C compared to 10 °C or the ambient temperature conditions of a glasshouse (maximum-minimum of 25–12.7 °C). A total of 49 embryos was obtained from 2660 cultured flower buds and 45% of plantlets were successfully acclimatised to glasshouse conditions. The majority of acclimatised plantlets were haploid (68%) but spontaneous double haploid plants (23%) were obtained from three genotypes.
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Puddephat, I., Robinson, H., Smith, B. et al. Influence of stock plant pretreatment on gynogenic embryo induction from flower buds of onion. Plant Cell, Tissue and Organ Culture 57, 145–148 (1999). https://doi.org/10.1023/A:1006312614874
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DOI: https://doi.org/10.1023/A:1006312614874