Abstract
Klebsiella pneumoniae, which produces PQQ and is available for use with a conventional expression vector system, was selected as the host strain for soluble PQQ glucose dehydrogenase (PQQGDH-B) production. The recombinant K. pneumoniaeexpressed PQQGDH-B in its holo-form at about 18 000 U l−1, equal to that achieved in recombinant Escherichia coli. The signal sequence of recombinant PQQGDH-B produced by K. pneumoniaewas correctly processed. K. pneumoniaecan become an alternative host microorganism not only for PQQGDH-B production but also for recombinant PQQ enzymes production.
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Cleton-Jansen AM, Goosen N, Fayet O, van de Putte P (1990) Cloning, mapping, and sequencing of the gene encording Escherichia coliquinoprotein glucose dehydrogenase. J. Bacteriol. 172: 6308-6315.
Cleton-Jansen AM, Goosen N, Wenzel TJ, van de Putte P (1988) Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme. J. Bacteriol. 170: 2121-2125.
Harding NE, Cleary JM, Smith DW, Michon JJ, Brusilow WSA, Wyskind JW (1982) Chromosomal Replication origins (OriC) of Enterobacter aerogenesand Klebsiella pneumoniaeare functional in Escherichia coli. J. Bacteriol. 152: 983-993.
Kleiner D, Paul W, Merrick MJ (1988) Construction of multicopy expression vectors for regulated overproduction of proteins in Klebsiella pneumoniaeand other enteric bacteria. J. Gen. Microbiol. 134: 1779-1784.
Kost GJ, Vu HT, Lee JH, Bourgeois P, Kiechle FL, Martin C, Miller SS, Okorodudu AO, Podczasy JJ, Webster R, Whitlow KJ (1998) Multicenter study of oxygen-insensitive handheld glucose pointof-care testing in critical care/hospital/ambulatory patients in the United States and Canada. Crit. Care. Med. 26: 581-590.
Matsushita K, Toyama H, Ameyama M, Adachi O, Dewanti A, Duine JA (1995) Soluble and membrane-bound quinoprotein D-glucose dehydrogenase of Acinetobacter calcoaceticus: the binding process of PQQ to the apoenzymes. Biosci. Biotech. Biochem. 59: 1548-1555.
Meulenberg JJM, Sellink E, Loenen WAM, Riegman NH, van Kleef M, Postma PW (1990) Cloning of Klebsiella pneumoniae pqqgenes and PQQ biosyanthesis in Escherichia coli. FEMS Microbiol. Lett. 71: 337-344.
Neijssel OM, Tempest DW, Postma PW, Duine JA, Frank JznJ (1983) Glucose metabolism by KC-limited Klebsiella aerogenes, evidence for involvement of a quinoprotein glucose dehydrogenase. FEMS Microbiol. Lett. 20: 35-39.
Robinson A, Tempest DW (1973) Phenotypic variability of the envelope proteins of Klebsiella aerogenes. J. Gen. Microbiol. 78: 361-370.
Schmidt B (1997) Oxygen-independent oxidases: a new class of enzymes for application in diagnostics. Clin. Chim. Acta 266: 33-37.
Sode K, Ito K, Witarto AB, Watanabe K, Yoshida H, Postma P (1996) Increased production of recombinant pyrroloquinoline quinone (PQQ) glucose dehydrogenase by metabolically engineered Escherichia colistrain capable of PQQ biosynthesis. J. Biotechnol. 49: 239-243.
Sode K, Witarto AB, Watanabe K, Noda K, Ito S, Tsugawa W (1994) Over expression of PQQ glucose dehydrogenase in Escherichia coliunder holo-enzyme forming condition. Biotechnol. Lett. 16: 1265-1268.
Ye L, Haemmerle M, Olsthoom AJJ, Schumann W, Schmidt HL, Duine JA, Heller A (1993) High current density 'wired' quinoprotein glucose dehydrogenase electorode. Anal. Chem. 65: 238-241.
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Kojima, K., Witarto, A.B. & Sode, K. The production of soluble pyrroloquinoline quinone glucose dehydrogenase by Klebsiella pneumoniae, the alternative host of PQQ enzymes. Biotechnology Letters 22, 1343–1347 (2000). https://doi.org/10.1023/A:1005615400089
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DOI: https://doi.org/10.1023/A:1005615400089