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An alkalophilic thermostable lipase produced by a new isolate of Bacillus alcalophilus

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Abstract

An extremophilic bacterium, isolated from mangrove detritus, produced an extracellular alkaline-thermostable lipase. The bacterium was identified on the basis of cell morphology, growth characteristics, G + C molar ratio and DNA/DNA hybridization as a strain of Bacillus alcalophilus. The bacterium grew optimally at pH 10.6, 60 °C with NaCl tolerance up to 7.5% (w/v). Carbonates and/or bicarbonates enhanced lipase production, while NaCl had an inhibitory effect. Maximum lipase activity was at 60 °C at pH 10.6, with approx. 60% of its activity being retained at 80 °C after 20 min and 80% of its activity was retained at pH 11 after incubation at 60 °C. A partially purified lipase had similar stabilities to the crude enzyme.

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Authors and Affiliations

  1. Department of Biology, College of Science, University of Bahrain, PO Box 32038, State of Bahrain

    Essam H. Ghanem, Hashim A. Al-Sayed & Kareema M. Saleh

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  1. Essam H. Ghanem
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  2. Hashim A. Al-Sayed
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  3. Kareema M. Saleh
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Ghanem, E.H., Al-Sayed, H.A. & Saleh, K.M. An alkalophilic thermostable lipase produced by a new isolate of Bacillus alcalophilus. World Journal of Microbiology and Biotechnology 16, 459–464 (2000). https://doi.org/10.1023/A:1008947620734

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