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Combined effect of cytokinins on multiple shoot production from cotyledonary node explants of Bauhinia vahlii

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Abstract

Using seedling explants, an improved regeneration protocol was developed for Bauhinia vahlii. A combination of thidiazuron and kinetin (1.0 μM each) increased the number of shoots significantly (p > 0.05) up to four successive subculture cycles. Over 83% shoots rooted on one-fourth strength Murashige and Skoog (MS) medium supplemented with 1.0 μM α-naphthaleneacetic acid (NAA). Fifty percent of plantlets (15 No.) successfully acclimatized in 90 g (w/v) soilrite + sand + soil (2:1:1) in the shed house. Preconditioning at different sucrose concentrations prior to acclimatization showed no effect on percent survival but improved plant quality.

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Authors and Affiliations

  1. G.B. Pant Institute of Himalayan Environment & Development, Kosi-Katarmal, Almora, 263 643 (U.P.), India

    Indra D. Bhatt, Uppeandra Dhar & Uppeandra Dhar

Authors
  1. Indra D. Bhatt
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  2. Uppeandra Dhar
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Correspondence to Uppeandra Dhar.

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Bhatt, I.D., Dhar, U. Combined effect of cytokinins on multiple shoot production from cotyledonary node explants of Bauhinia vahlii. Plant Cell, Tissue and Organ Culture 62, 79–83 (2000). https://doi.org/10.1023/A:1006450516329

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  • DOI: https://doi.org/10.1023/A:1006450516329

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