Skip to main content
SpringerLink
Log in

Plant regeneration from seedling explants of Eucalyptus grandis × E. urophylla

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Hypocotyls, cotyledons, cotyledonary nodes and primary leaves were used as explants to establish a regeneration protocol for Eucalyptus grandis × E. urophylla. These seedling-derived explants were incubated on a modified MS medium (SP medium), supplemented with 2.0 μM TDZ. After 1 month, the calluses obtained were transferred to SP medium containing different concentrations of BA and NAA or zeatin and NAA. Shoots were induced from these calluses at a high frequency. Shoot elongation was then stimulated on SP medium supplemented with BA, NAA and GA3 for 20 to 30 days. For rooting, 50 mm long shoots were cultivated on root induction medium containing IBA (2.5 μM) for different periods and then transferred to the same medium but without auxin, for 30 days. Plantlets were then successfully transplanted to greenhouse conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barrueto Cid LP, Illg RD & Piedrabuena AE (1994) Regeneration of garlic plants (Allium sativum L., cv.' chonan') via cell culture in liquid medium. In Vitro Cell Dev. Biol. 30P: 150–155

    Google Scholar 

  • Barrueto Cid LP, Gomes ACM, Costa SBR & Teixeira JB (1997) Micropropagation of Miconia sp, a woody Melastomaceae from Brazil, using thidiazuron as plant growth regulator. R. Bras. Fisiol. Veg. 9: 21–25

    Google Scholar 

  • Das T & Mitra GC (1990) Micropropagation of Eucalyptus tereticornis Smith. Plant Cell Tiss. Org. Cult. 22: 95–103

    Google Scholar 

  • Durand-Cresswell R, Boulay M & Franclet A (1985) Vegetative propagation of Eucalyptus. In: Bonga JM & Durzan DJ (eds) Tissue Culture in Forestry (pp 150–181). Martinus Nijhoff Publ., Dordrecht

    Google Scholar 

  • Ferreira M & Santos PET (1997) Genetic improvement of Eucalyptus in Brazil - brief review and perspectives. In: Proceedings IUFRO, Conference on Silviculture and Improvement of Eucalypt (pp 14–34). Salvador (Brazil)

    Google Scholar 

  • Gupta PK, Mehta UJ & Mascarenhas AF (1983) A tissue culture method for rapid clonal propagation of mature trees of Eucalyptus torelliana and Eucalyptus camaldulensis. Plant Cell Rep. 2: 296–299

    Google Scholar 

  • Haines R (1994) Biotechnology in Forest Tree Improvement. FAO, Rome

    Google Scholar 

  • Horgan K (1987) Pinus radiate. In: Bonga JM & Durzan DJ (eds) Cell and Tissue Culture in Forestry, Vol 3(pp 128–45). Martinus Nijhoff Publ., Dordrecht

    Google Scholar 

  • Huetteman CA & Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119

    Google Scholar 

  • Ikemori YK, Penchel RM & Bertolucci FLG (1994) Integrating biotecnology into eucalypt breeding. In: Proceedings International Wood Biotechnology Symposium (pp 77–84). Tokio (Japan)

    Google Scholar 

  • Jouanin L, Brasileiro ACM, Leplé JC, Pilate G & Corn D (1993) Genetic transformation: a short review of methods and their applications, results and perspectives for forest trees. Ann. Sci. For. 50: 325–336

    Google Scholar 

  • Lainé E & David A (1994) Regeneration of plants from leaf explants of micropropagated clonal Eucalyptus grandis. Plant Cell Rep. 13: 473–476

    Google Scholar 

  • Le Roux JJ & van Staden J (1991) Micropropagation of Eucalyptus species. Hortscience 26: 199–200

    Google Scholar 

  • Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev. Biol. 29P: 92–96

    Google Scholar 

  • Machado LOR, Andrade GM, Barrueto Cid LP, Penchel RM & Brasileiro ACM (1997) Agrobacterium strain specificity and shooty tumour formation in eucalypt (Eucalyptus grandis x E. urophylla). Plant Cell Rep. 16: 299–303

    Google Scholar 

  • Mayer AM (1987) Polyphenol oxidases in plants - recent progress. Phytochemistry 26: 11–20

    Article  Google Scholar 

  • Mullins KV, Llewellyn DJ, Hartney VJ, Strauss S & Dennis ES (1997) Regeneration and transformation of Eucalyptus camaldulensis. Plant Cell Rep. 16: 787–791

    Google Scholar 

  • Muralidharan EM & Mascarenhas AF (1987) In vitro plantlet formation by organogenesis in E.camaldulensis and by somatic embryogenesis in Eucalyptus citriodora. Plant Cell Rep. 6: 256–259

    Article  Google Scholar 

  • Muralidharan EM, Gupta PK & Mascarenhas AF (1989) Plantlet production through high frequency somatic embryogenesis in long term cultures of Eucalyptus citriodora. Plant Cell Rep. 8: 41–43

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Serrano L, Rochange F, Semblat JP, Marque C, Teulières C & Boudet AM (1996) Genetic transformation of Eucalyptus globulus through biolistics: complementary development of procedures for organogenesis from zygotic embryos and stable transformation of corresponding proliferating tissue. J. Exp. Botany 47: 285–290

    Google Scholar 

  • Subbaiah MM & Minocha SC (1990) Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis. Plant Cell Rep. 9: 370–373

    Google Scholar 

  • Termignoni RR, Wang PJ & Hu CY (1996) Somatic embryo induction in Eucalyptus dunnii. Plant Cell Tiss. Org. Cult. 45: 129–132

    Google Scholar 

  • Teulières C, Marque C & Boudet AM(1994) Genetic transformation of Eucalyptus. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 29(pp 289–307). Springer-Verlag, Berlin

    Google Scholar 

  • Tibok A, Blackhall NW, Power JB & Davey MR (1995) Optimized plant regeneration from callus derived from seedling hypocotyls of Eucalyptus urophylla. Plant Sci. 110: 139–145

    Google Scholar 

  • Warrag E, Lesney MS & Rockwood DJ (1991) Nodule culture and regeneration of Eucalyptus grandis hybrids. Plant Cell Rep. 9: 586–589

    Google Scholar 

  • Watt MP, Blakeway F, Cresswell CF & Herman B (1991) Somatic embryogenesis in Eucalyptus grandis. South African For. J. 157: 59–65

    Google Scholar 

  • Zambre MA, De Clercq J, Vranová E, Van Montagu M, Angenon G & Dillen W (1998) Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P.acutifolius A. Gray (tepary bean). Plant Cell Rep. 17: 626–630

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Área de Biologia Celular – Cenargen/Embrapa, C.P. 02372, CEP 70.849-970, Brasília-DF, Brazil requests for offprints; fax

    Luis Pedro Barrueto Cid, Adriane C.M.G. Machado, Sílvia B.R.C. Carvalheira & Ana Cristina M. Brasileiro

Authors
  1. Luis Pedro Barrueto Cid
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adriane C.M.G. Machado
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sílvia B.R.C. Carvalheira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ana Cristina M. Brasileiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luis Pedro Barrueto Cid.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barrueto Cid, L.P., Machado, A.C., Carvalheira, S.B. et al. Plant regeneration from seedling explants of Eucalyptus grandis × E. urophylla. Plant Cell, Tissue and Organ Culture 56, 17–23 (1999). https://doi.org/10.1023/A:1006283816625

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006283816625

Search

Navigation