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Somatic embryogenesis and plant regeneration in Anthurium scherzerianum

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Abstract

Several types of explants, growth regulators, sugars and gelling agents were tested to induce somatic embryogenesis in Anthurium scherzerianum Schott. For induction, leaf pieces from micropropagated plants were found to be the best explant on a medium with 18 μM 2,4-dichlorophenoxyacetic acid and a high sucrose concentration (6), solidified with Gelrite. Somatic embryos converted to entire plants on a medium with 0.46 μM kinetin. Plantlets were transferred to greenhouse conditions for evaluation of plant uniformity.

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References

  • DeWald SG, Litz RE & Moore GA (1989) Optimizing somatic embryo production in Mango. J. Amer. Soc. Hort. Sci. 114: 712–716

    Google Scholar 

  • Geier T (1982) Morphogenesis and plant regeneration from spadix fragments of Anthurium scherzerianum. In: Fujiwara A (ed) Plant Tissue Culture 1982 (pp 137–138). Japanese Association for Plant Tissue Culture, Tokyo, Japan

    Google Scholar 

  • Geier T (1988) Ploidy variation in callus and regenerated plants of Anthurium scherzerianum Schott. Acta Hort. 226: 293–298

    Google Scholar 

  • Geier T (1990) Anthurium. In: Ammirato PV, Evans DA, Sharp WR & Bajaj YPS (ed) Handbook of Plant Cell Culture, Ornamental species, Vol 5 (pp 228–252). McGraw-Hill, New York

    Google Scholar 

  • Johansen DA(1940) Plant microtechnique. McGraw Hill, New York

    Google Scholar 

  • Kuehnle AR & Sugii N (1991) Callus induction and plantlet regeneration in tissue cultures of Hawaiian Anthuriums. HortSci. 26: 919–921

    Google Scholar 

  • Kuehnle AR, Chen F & Sugii N (1992) Somatic embryogenesis and plant regeneration in Anthurium andraeanum hybrids. Plant Cell Rep. 11: 438–442

    Google Scholar 

  • Lamproye A, Hofinger M, Berthon J & Gaspar J (1990) [Benzo (b) selenienyl-3] acetic acid: a potent synthetic auxin in somatic embryohenesis. C. R. Acad. Sci. Paris, t. 311, Serie III, 127–132

    CAS  Google Scholar 

  • Levi A & Sink KC (1990) Differential effects of sucrose, glucose and fructose during somatic embryogenesis in asparagus. J. Plant Physiol. 137: 184–189

    CAS  Google Scholar 

  • McCown BH, Zeldin EL, Pinkala HA & Dedolph RR (1988) Nodule culture: A developmental pathway with high potential for regeneration, automated micropropagation, and plant metabolite production from woody plants. In: Hanover JW & Keathley DE (ed) Genetic Manipulation of Woody Plants (pp 149–166). Plenum Press, New York and London

    Google Scholar 

  • Merkle SA, Parrot WA & Williams EG (1990) Applications of somatic embryogenesis and embryo cloning. In: Bhojwani SS (ed) Developments in Crop Science 19. Plant Tissue Culture: Applications and Limitations (pp 67–101). Elsevier. Amsterdam-Oxford-Tokyo

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Article  CAS  Google Scholar 

  • Pierik RLM, Steegmans HHM & Van Der Meys JAJ (1974) Plantlet formation in callus tissues of Anthurium andraeanum Lind. Sci. Hort. 2: 193–198

    Article  Google Scholar 

  • Trolinder NL & Goodin JR (1988) Somatic embryogenesis in cotton (Gossypium) I. Effects of source of explant and hormone regime. Plant Cell Tiss. Org. Cult. 12: 31–42

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Dept. Plant Production - Horticulture, University Gent, Coupure Links 653, B9000, Gent, Belgium

    Musa Hamidah & Pierre Debergh

  2. Dept. Botany, Faculty of life Sciences, National University of Malaysia, 43600, Bangi, Selangor, Malaysia

    Abdul Ghani Abdul Karim

Authors
  1. Musa Hamidah
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  2. Abdul Ghani Abdul Karim
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  3. Pierre Debergh
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Hamidah, M., Karim, A.G.A. & Debergh, P. Somatic embryogenesis and plant regeneration in Anthurium scherzerianum . Plant Cell, Tissue and Organ Culture 48, 189–193 (1997). https://doi.org/10.1023/A:1005834131478

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  • DOI: https://doi.org/10.1023/A:1005834131478

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