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Improved long-term cryostorage of Escherichia colicompetent cells using trehalose

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Abstract

Trehalose added to storage buffers for chemical-induced transformations at 200 mm increased the survival of Escherichia coli competent cells 1.3∼4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.

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Authors and Affiliations

  1. Department of Biochemistry, College of Veterinary Medicine, Korea

    Taeho Ahn

  2. Interdisciplinary Program of Brain Science, Chonnam National University, Gwangju, 500-757, Korea

    Seong Su Kang

  3. School of Biological Sciences and Technology, Chonnam National University, Gwangju, 500-757, Korea

    Chul-Ho Yun

Authors
  1. Taeho Ahn
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  2. Seong Su Kang
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  3. Chul-Ho Yun
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Ahn, T., Kang, S.S. & Yun, CH. Improved long-term cryostorage of Escherichia colicompetent cells using trehalose. Biotechnology Letters 26, 1593–1594 (2004). https://doi.org/10.1023/B:BILE.0000045659.41621.de

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  • DOI: https://doi.org/10.1023/B:BILE.0000045659.41621.de

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