Abstract
Changes in the molecular structure of cellulose during hydrolysis with four recombinant β-1,4-glycanases from the cellulolytic bacterium Cellulomonas fimi were assessed and compared in an attempt to elucidate the mechanism of crystalline cellulose degradation. It was apparent that the two endoglucanases, Cel6A and Cel5A, degraded sigmacell cellulose differently; Cel5A liberated more soluble sugars (cellobiose and cellotriose) and significantly altered the molecular weight distribution, while Cel6A had a limited effect on the polymer size and liberated primarily cellobiose and glucose. Additionally, both endoglucanases slightly increased the crystallinity of cellulose. In contrast, the cellobiohydrolases, Cel6B and Cel48A, had no effect on cellulose molecular weight and liberated only cellobiose and cellotriose. However, Cel48A was shown to be effective at reducing the crystallinity of the cellulosic substrate, while Cel6B increased the crystallinity index. Synergistic hydrolysis using combinations of the different enzymes showed that, although the cellulose was extensively hydrolysed, the molecular structure of the substrate was similar to the original material. This phenomenon suggests that the actions of individual monocomponent enzymes are offset by the concurrent modification by the complementing enzymes during synergistic hydrolysis.
Similar content being viewed by others
References
Atalla R.H. 1993. The Structures of Native Celluloses. Foundation for Biotechnical and Industrial Fermentation Research, Espoo, Finland.
Carrard G., Koivula A., Söderlund H. and Béguin P. 2000. Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl. Acad. Sci. USA 97: 10342-10347.
Chanzy H. and Henrissat B. 1985. Undirectional degradation of Valonia cellulose microcrystals subjected to cellulase action. FEBS Lett. 184: 285-288.
Fan L.T., Lee Y.-H. and Beardmore D.H. 1980. Mechanism of the enzymatic hydrolysis of cellulose: effects of major structural features of cellulose on enzymatic hydrolysis. Biotechnol. Bioeng. 22: 177-199.
Fan L.T., Lee Y.-H. and Beardmore D.R. 1981. The influence of major structural features of cellulose on rate of enzymatic hydrolysis. Biotechnol. Bioeng. 23: 419-424.
Gilkes N.R., Kwan E., Kilburn D.G., Miller R.C. and Warren R.A.J. 1997. Attack of carboxymethylcellulose at opposite ends by two cellobiohydrolases from Cellulomonas fimi. J. Biotechnol. 57: 83-90.
Gilkes N.R., Warren R.A.J., Miller R.C. Jr. and Kilburn D.G. 1988. Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis. J. Biol. Chem. 263: 10401-10407.
Gübitz G.M., Mansfield S.D., Böhm D. and Saddler J.N. 1998. Effect of endoglucanases and hemicellulases in magnetic and floatation deinking of xerographic and laser-printed papers. J. Biotechnol. 65: 209-219.
Imai T., Boisset C., Samejima M., Igarashi K. and Sugiyama J. 1998. Unidirectional processive action of cellobiohydrolase Cel7A on Valonia cellulose microcrystals. FEBS Lett. 432: 113-116.
Irwin D.C., Spezio M., Walker L.P. and Wilson D.B. 1993. Activity studies of eight purified cellulases: specificity, synergism, and binding domain effects. Biotechnol. Bioeng. 42: 1002-1013.
Kleman-Leyer K., Gilkes N.R., Miller R.C. Jr. and Kirk T.K. 1994. Changes in molecular size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases. Biochem. J. 302: 463-469.
Kleman-Leyer K.M., Siika-aho M., Teeri T.T. and Kirk T.K. 1996. The cellulases endoglucanase I and cellobiohydrolase II of Trichoderma reesei act synergistically to solubilize native cotton cellulose but not to decrease its molecular size. Appl. Environ. Microbiol. 62: 2883-2887.
Koyama M., Helbert W., Imai T., Sugiyama J. and Henrissat B. 1997. Parallel-up structure evidences the molecular directionality during biosynthesis of bacterial cellulose. Proc. Natl. Acad. Sci. USA 94: 9091-9095.
Mansfield S.D., Mooney C. and Saddler J.N. 1999. Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnol. Prog. 15: 804-816.
Mansfield S.D., Saddler J.N. and Gübitz G.M. 1998. Characterization of endoglucanases from the brown rot fungi Gloeophyllum sepiarium and Gloeophyllum trabeum. Enzyme Microb. Technol. 23: 133-140.
Meinke A., Gilkes N.R., Kilburn D.G., Miller J.R.C. and Warren R.A.J. 1993. Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A β-1,4-glucanase. J. Bacteriol. 175: 1910-1918.
Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J. and Miller R.C. Jr. 1994. Cellobiohydrolase A from the cellulolytic bacterium Cellulomonas fimi is a β-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II. Mol. Microbiol. 12: 413-422.
Michell A.J. 1989. Second derivative FTIR spectra of woods. In: Schuerch C. (ed.), Cellulose and Wood-Chemistry and Technology. John Wiley & Sons, Inc., New York, pp. 995-1009.
Newman R.H. and Hemmingson J.A. 1990. Determination of the degree of cellulose crystallinity in wood by carbon-13 nuclear magnetic resonance spectroscopy. Holzforschung 44: 351-355.
Nidetzky B., Hayn M., Macarron R. and Steiner W. 1993. Synergism of Trichoderma reesei cellulases while degrading different celluloses. Biotechnol. Lett. 15: 71-76.
Schroeder L.R. and Haigh F.C. 1979. Cellulose and wood pulp polysaccharides. Gel permeation chromatography analysis. Tappi 62: 103-105.
Shen H., Gilkes N.R., Kilburn D.G., Miller R.C. and Warren R.A.J. 1995a. Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi. Biochem. J. 311: 67-74.
Shen H., Meinke A., Tomme P., Damude H., Kwan E., Kilburn D.G. et al. 1995b. Cellulomonas fimi cellobiohydrolases. In: Saddler J.N. and Penner M.H. (eds), Enzymatic Degradation of Insoluble Carbohydrates. American Chemical Society Symposium Series. American Chemical Society, Washington, DC, pp. 174-196.
Srishdsuk M., Kleman-Leyer K., Keränen S., Kirk T.K. and Teeri T.T. 1998. Modes of action on cotton and bacterial cellulose of a homologous endoglucanase-exoglucanase pair from Trichoderma reesei. Eur. J. Biochem. 251: 885-892.
Stålbrand H., Mansfield S.D., Saddler J.N., Kilburn D.G., Warren R.A.J. and Gilkes N.R. 1998. Analysis of molecular size distributions of cellulose molecules during hydrolysis of cellulose by recombinant Cellulomonas fimi β-1,4-glucanases. Appl. Environ. Microbiol. 64: 2374-2379.
Teeri T.T. 1997. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Tibtech 15: 160-167.
Valtasaari L. and Saarela K. 1975. Determination of chain length distribution of cellulose by gel permeation chromatography using the tricarbanilate derivative. Paperi ja Puu-Papper och Trä 57: 5-10.
Walker L.P. and Wilson D.B. 1991. Enzymatic hydrolysis of cellulose: an overview. Biores. Technol. 36: 3-14.
Wood T.M. and McCrae S.I. 1979. Synergism between enzymes involved in the solubilization of native cellulose. Adv. Chem. Ser. 181: 181-209.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mansfield, S.D., Meder, R. Cellulose hydrolysis – the role of monocomponent cellulases in crystalline cellulose degradation. Cellulose 10, 159–169 (2003). https://doi.org/10.1023/A:1024022710366
Issue Date:
DOI: https://doi.org/10.1023/A:1024022710366