Abstract
The colonization ability of Pseudomonas fluorescens F113rif in alfalfa rhizosphere and its interactions with the alfalfa microsymbiont Sinorhizobium meliloti EFB1 has been analyzed. Both strains efficiently colonize the alfalfa rhizosphere in gnotobiotic systems and soil microcosms. Colonization dynamics of F113rif on alfalfa were similar to other plant systems previously studied but it is displaced by S. meliloti EFB1, lowering its population by one order of magnitude in co-inoculation experiments. GFP tagged strains used to study the colonization patterns by both strains indicated that P. fluorescens F113rif did not colonize root hairs while S. meliloti EFB1 extensively colonized this niche. Inoculation of F113rif had a deleterious effect on plants grown in gnotobiotic systems, possibly because of the production of HCN and the high populations reached in these systems. This effect was reversed by co-inoculation. Pseudomonas fluorescens F113 derivatives with biocontrol and bioremediation abilities have been developed in recent years. The results obtained support the possibility of using this bacterium in conjunction with alfalfa for biocontrol or rhizoremediation technologies.
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References
Bakker AW and Schippers B 1987 Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant-growth stimulation. Soil. Biol. Biochem. 19, 451–457.
Barea J M, Andrade G, Bianciotto V, Dowling D, Lohrke S, Bonfante P, O'Gara F and Azcon-Aguilar C 1998 Impact on arbuscular mycorrhiza formation of Pseudomonas strains used as inoculants for biocontrol of soil-borne fungal plant pathogens. Appl. Environ. Microbiol. 64, 2304–2307.
Brazil G M, Kenefick L, Callanan M, Haro A, de Lorenzo V, Dowling D N and O'Gara F 1995 Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere Appl. Environ. Microbiol. 61, 1946–1952.
Chabot R, Antoun H, Kloepper J W and Beauchamp C J 1996 Root colonization of maize and lettuce by bioluminescent Rhizobium leguminosarum biovar phaseoli. Appl. Environ. Microbiol. 62, 2767–2772.
Cheng H P and Walker G C 1998. Succinoglycan is required for initiation and elongation of infection threads during nodulation of alfalfa by Rhizobium meliloti. J. Bacteriol. 180, 5183–5191.
Chin-A-Woeng T F C, de Priester W, van der Bij A and Lugtenberg B 1997 Description of the colonization of a gnotobiotic tomato rhizosphere by Pseudomonas fluorescens biocontrol strain WCS365, using scanning electron microscopy. Mol. Plant-Microbe Interact. 10, 79–86.
Chin-A-Woeng T F C, Bloemberg G V, Mulders I H M, Dekkers L C and Lugtenberg B J J 2000 Root colonization by phenazine-1-carboxamide-producing bacterium Pseudomonas chlororaphis PCL1391 is essential for biocontrol of tomato foot and root rot. Mol Plant-Microbe Interact 13, 1340–1345.
de Weger L A, van der Vlugt C I, Wijfjes A H, Bakker P A, Schippers B and Lugtenberg B 1987 Flagella of a plant-growth stimulating Pseudomonas fluorescens strain are required for colonization of potato roots. J. Bacteriol. 169, 2769–2773.
Dekkers L C, van der Bij A J, Mulders I H, Phoelich C C, Wentwoord R A, Glandorf D C, Wijffelman C A and Lugtenberg B 1998 Role of the O-antigen of lipopolysaccharide, and possible roles of growth rate and of NADH:ubiquinone oxidoreductase (nuo) in competitive tomato root-tip colonization by Pseudomonas fluorescens WCS365. Mol. Plant-Microbe Interact. 11, 763–771.
Delany I R, Walsh U F, Ross I, Fenton A M, Corkery D M and O'Gara F 2001 Enhancing the biocontrol efficacy of Peudomonas fluorescens F113 by altering the regulation and production of 2,4-diacetylphloroglucinol. Plant Soil 232, 195–205.
Dowling D N and O'Gara F 1994 Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends Biotechnol. 12, 133–141.
Fåhraeus G 1957 The infection of clover root hairs by nodule bacteria studied by simple glass technique. J. Gen. Microbiol. 16, 374–381.
Fedi S, Brazil D, Dowling D N and O'Gara F 1996 Construction of a modified mini-Tn5 lacZY non-antibiotic marker cassette: ecological evaluation of a lacZY marked Pseudomonas strain in the sugarbeet rhizosphere. FEMS Microbiol. Lett. 135, 251–257.
Figurski D H and Helinski D R 1979 Replication of an origincontaining derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76, 1648–1652.
Hofer B, Backhaus S and Timmis K N 1994 The biphenyl/ polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymes. Gene 144, 9–16.
Höflich G, Wiehe W and Hecht-Buchholz C 1995 Rhizosphere colonization of different crops with growth promoting Pseudomonas and Rhizobium bacteria. Microbiol. Res. 150, 139–147.
Karlson U, Dowling D N, O'Gara F, Rivilla R, Bittens M, Francesconi S, Pritchard H C and Pedersen H C 1998 Development of Self-Contained Plant/GMM Systems for Soil Bioremediation. Past, Present and Future Risk Assessment When Using GMO's. Gert E. de Vries. ProBio Partners V.O.F., Overschild, The Netherlands, pp. 23–31.
Lloret J, Bolaños L, Lucas M M, Peart J M, Brewin N J, Bonilla I and Rivilla R 1995 Ionic stress and osmotic pressure induce different alterations in the lipopolysaccharide of a Rhizobium meliloti strain. Appl. Environ. Microbiol. 61, 3701–3704.
Lugtenberg B J J and Dekkers L C 1999 What makes Pseudomonas bacteria rhizosphere competent? Environ. Microbiol. 1, 9–13.
Naseby D C and Lynch J M 1999 Effects of Pseudomonas fluorescens F113 on ecological functions in the pea rhizosphere are dependent on pH. Microb. Ecol. 37, 248–256.
Normander B, Hendriksen N B and Nybroe O 1999 Green fluorescent protein-marked Pseudomonas fluorescens: localization, viability, and activity in the natural barley rhizosphere. Appl. Environ. Microbiol. 65, 4646–4651.
Schloter M, Wiehe W, Assmus B, Steindl H, Becke H, Höflich G and Hartmann A 1997 Root colonization of different plants by plant-growth-promoting Rhizobium leguminosarum bv. trifolii R39 studied with monospecific polyclonal antisera. Appl. Environ. Microbiol. 63, 2038–2046.
Shanahan P, O'Sullivan D J, Simpson P, Glennon J D and O'Gara F 1992 Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Appl. Environ. Microbiol. 53, 353–358.
Simons M, van der Bij A J, Brand I, de Weger L A, Wijffelman C A and Lugtenberg B J J 1996 Gnotobiotic system for studying rhizosphere colonization by plant growth-promoting Pseudomonas bacteria. Mol. Plant-Microbe Interact. 9, 600–607.
Tombolini R, van der Gaag D J, Gerhardson B and Jansson J K 1999 Colonization pattern of the biocontrol strain Pseudomonas chlororaphis MA 342 on barley seeds visualized by using green fluorescent protein. Appl. Environ. Microbiol. 65, 3674–3680.
Yee D C, Maynard J A and Wood T K 1998 Rhizoremediation of trichloroethylene by a recombinant, root-colonizing Pseudomonas fluorescens strain expressing toluene ortho-monooxygenase constitutively. Appl. Environ. Microbiol. 64, 112–118.
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Villacieros, M., Power, B., Sánchez-Contreras, M. et al. Colonization behaviour of Pseudomonas fluorescens and Sinorhizobium meliloti in the alfalfa (Medicago sativa) rhizosphere. Plant and Soil 251, 47–54 (2003). https://doi.org/10.1023/A:1022943708794
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DOI: https://doi.org/10.1023/A:1022943708794