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Beneficial native bacteria improve survival and mycorrhization of desert truffle mycorrhizal plants in nursery conditions

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Abstract

Sixty-four native bacterial colonies were isolated from mycorrhizal roots of Helianthemum almeriense colonized by Terfezia claveryi, mycorrhizosphere soil, and peridium of T. claveryi to evaluate their effect on mycorrhizal plant production. Based on the phylogenetic analysis of the 16S rDNA partial sequence, 45 different strains from 17 genera were gathered. The largest genera were Pseudomonas (40.8 % of the isolated strains), Bacillus (12.2 % of isolated strains), and Varivorax (8.2 % of isolated strains). All the bacteria were characterized phenotypically and by their plant growth-promoting rhizobacteria (PGPR) traits (auxin and siderophore production, phosphate solubilization, and ACC deaminase activity). Only bacterial combinations with several PGPR traits or Pseudomonas sp. strain 5, which presents three different PGPR traits, had a positive effect on plant survival and growth. Particularly relevant were the bacterial treatments involving auxin release, which significantly increased the root-shoot ratio and mycorrhizal colonization. Moreover, Pseudomonas mandelii strain 29 was able to considerably increase mycorrhizal colonization but not plant growth, and could be considered as mycorrhiza-helper bacteria. Therefore, the mycorrhizal roots, mycorrhizosphere soil, and peridium of desert truffles are environments enriched in bacteria which may be used to increase the survival and mycorrhization in the desert truffle plant production system at a semi-industrial scale.

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Acknowledgments

This work was supported by project CGL2011-29816 (MINECO-FEDER, Spain) and project 19484/PI/14 (FEDER-Programa de Apoyo a la Investigación de la Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia, Spain). A. Navarro-Ródenas is grateful to MINECO for a postdoctoral Torres-Quevedo contract PTQ-12-05818. The authors thank JJ Bordallo for his help with DNA sequence identification and Dr. J. Zwiazek for his helpful comments on the manuscript.

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Authors and Affiliations

  1. Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain

    Cecilia Lozano-Carrillo & Asunción Morte

  2. Thader Biotechnology SL, Ed. Parque Científico 6, Campus de Espinardo, 30100, Murcia, Spain

    Alfonso Navarro-Ródenas, Luis Miguel Berná, Alberto Andrino & Asunción Morte

  3. Institute of Soil Science, Leibniz Universität Hannover, 30419, Hanover, Germany

    Alberto Andrino

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  1. Alfonso Navarro-Ródenas
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  2. Luis Miguel Berná
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  3. Cecilia Lozano-Carrillo
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  4. Alberto Andrino
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  5. Asunción Morte
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Correspondence to Asunción Morte.

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Supplementary Table 1. Phenotypic characteristics of the isolated bacterial strains. (DOCX 22 kb)

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Navarro-Ródenas, A., Berná, L.M., Lozano-Carrillo, C. et al. Beneficial native bacteria improve survival and mycorrhization of desert truffle mycorrhizal plants in nursery conditions. Mycorrhiza 26, 769–779 (2016). https://doi.org/10.1007/s00572-016-0711-6

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