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Effect of Dose Rate of Azoxystrobin and Metconazole on the Development of Fusarium Head Blight and the Accumulation of Deoxynivalenol (DON) in Wheat Grain

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Abstract

Glasshouse studies were undertaken to determine if fungicides used for the control of Fusarium head blight (FHB) result in elevated concentrations of the trichothecene mycotoxin, deoxynivalenol (DON) in harvested wheat grain. Metconazole and azoxystrobin, at double, full, half or quarter the manufacturer's recommended dose rate, were applied to ears of wheat (cv. Cadenza), artificially inoculated with conidia of either Fusarium culmorum or F. graminearum. Metconazole demonstrated high activity against both pathogens, reducing significantly the severity of FHB and the DON concentrations at each of the four dose rates tested when compared to untreated controls. Applications of azoxystrobin significantly reduced FHB and DON compared to unsprayed controls. However, their effectiveness was significantly less than that of metconazole and no dose rate response was observed. Quantification of the amount of trichothecene-producing Fusarium present in harvested grain was determined using a competitive PCR assay based on primers derived from the trichodiene synthase gene (Tri5). Simple linear regression analyses revealed strong relationships between the amount of trichothecene-producing Fusarium present in grain and the DON concentrations (r 2=0.72–0.97). It is concluded that fungicides, applied for the control of FHB, affect DON concentrations indirectly by influencing the amount of trichothecene-producing Fusarium species present in wheat grain. There was no evidence that fungicide applications directly increase the concentration of DON in grain.

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References

  • Apony I, Nagy G, Princzinger G and Kajati I (1998) Fusarium infection of wheat seeds in Hungary between 1970 and 1997. Cereal Research Communication 26: 253–258

    Google Scholar 

  • Bhat RV, Beedu SR, Ramakrishna Y and Munshi KL (1989) Outbreak of trichothecene mycotoxicosis associated with consumption of mould-damaged wheat products in Kashmir Valley, India. The Lancet 7: 35–37

    Google Scholar 

  • Boyacioglu D, Hettiarachchy NS and Stack RW (1992) Effects of three systemic fungicides on deoxynivalenol (vomitoxin) production by Fusarium graminearum in wheat. Canadian Journal of Plant Science 72: 93–101

    Google Scholar 

  • Birzele B, Prange A and Kramer J (2000) Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters. Food Additives and Contaminants 17: 1027–1035

    Google Scholar 

  • Chelkowski J (1989) Fusarium: Mycotoxins, Taxonomy and Pathogenicity. Elsevier Science Publishing Co., Amsterdam, The Netherlands

    Google Scholar 

  • Chelkowski J, Wishniewska H, Adamski T, Golinski P, Kaczmarek Z, Kostecki M, Perkowski J and Surma M (2000) Effect of Fusarium culmorum head blight on mycotoxin accumulation and yield traits in barley doubled haploids. Journal of Phytopathology 148: 541–545

    Google Scholar 

  • Dardis J and Walsh EJ (2000) Studies of the effectiveness of metconazole in controlling Fusarium Head Blight caused by Fusarium culmorum in spring wheat (Triticum aestivum L.). Cereal Research Communications 28: 443–448

    Google Scholar 

  • Edwards SG, Pirgozliev SR, Hare MC and Jenkinson P (2001) Quantification of trichothecene-producing Fusarium species in harvested grain by competitive PCR to determine the efficacy of fungicides against Fusarium Head Blight of winter wheat. Applied and Environmental Microbiology 67: 1575–1580

    Google Scholar 

  • Ellner FM and Schroer R (2000) Effects of fungicides containing strobilurin on mycotoxin production in wheat. In: Proceedings of the 6th European Fusarium Seminar, 11-16 September 2000, (p 101) Berlin, Germany

  • Gareis M and Ceynova J (1994) Influence of the fungicide Matador (tebuconazole/triadimenol) on mycotoxin production by Fusarium culmorum. Zeitschrift fur Lebensmittel-Untersuchung und - Forschung 198: 244–248

    Google Scholar 

  • Hart P and Ward R (1997) Efficacy of fungicides on Fusarium head blight severity and levels of vomitoxin. In: Proceedings of National Fusarium Head Blight Forum, 10-12 November 1997, (pp 40–41) St. Paul, Minn

  • Hoerr RJ, Carlton WW, Yagan B and Joffe AZ (1982) Mycotoxicosis produced in broiler chickens by multiple doses of either T-2 toxin or diacetoxyscirpenol. Avian Pathology 11: 369–383

    Google Scholar 

  • Homdork S, Fehermann H and Beck R (2000) Influence of different storage conditions on the mycotoxin production and quality of Fusarium-infected wheat grain. Journal of Phytopathology 148: 7–15

    Google Scholar 

  • Ichinoe M and H Kurata (1983) Trichothecene-producing fungi. In: Ueno Y (ed) Trichothecenes - Chemical, Biological and Toxicological Aspects (pp 73–82) Kodansha Ltd, Tokyo

    Google Scholar 

  • Jennings P, Turner JA and Nicholson P (2000) Overview of Fusarium Ear Blight in the UK - effect of fungicide treatment on disease control and mycotoxin production. In: Proceedings of The Brighton Crop Protection Conference - Pests and Diseases (pp 707–712) British Crop Protection Council, Farham, UK

    Google Scholar 

  • Joffe AZ (1978) Fusarium poae and Fusarium sporotrichoides as principal casual agents of alimentary toxic aleucia. In: Willie RD and Morehouse LR (eds) Mycotoxic Fungi, Mycotoxins, Mycotoxicoses - An Encyclopaedic Handbook, Vol 3 (pp 21–86) Marcel Dekker, New York

    Google Scholar 

  • Jones RK (2000) Assessments of Fusarium Head Blight of wheat and barley in response to Fungicide treatment. Plant Disease 84: 1021–1030

    Google Scholar 

  • Jones DR, Shearman V and Sylvester-Bradley R (2001) Understanding the effects of new wheat fungicides on disease development, crop growth and yield.Home Grown Cereals Authority Report No. 261, September 2001

  • Li QF, Luo XY and Yoshizava T (1999) Mycotoxins (Trichothecenes, Zearalenone and Fumonisins) in cereals associated with human red-mold intoxications stored since 1989 and 1991 in China. Natural Toxins 7: 93–97

    Google Scholar 

  • Long GG, Diekman M, Tutie JF, Shannon GM and Vesonder RF (1982) Effects of Fusarium roseum corn culture containing zearalenone on early pregnancy in swine. American Journal of Veterinary Research 43: 1599–1603

    Google Scholar 

  • Martin RA and Johnston HW (1982) Effects and control of Fusarium diseases of cereal grains in the Atlantic Provinces. Canadian Journal of Plant Pathology 4: 210–216

    Google Scholar 

  • Matthies A and Buchenauer H (2000) Effect of tebuconazole (Folicur®) and prochloraz (Sportac®) treatments on Fusarium Head Scab development, yield and deoxynivalenol (DON) content in grains of wheat following artificial inoculation with Fusarium culmorum. Journal of Plant Disease and Protection 107: 33–52

    Google Scholar 

  • Milus EA and Weight CT (1998) Efficacy of Quadris and Benlate Applications on Wheat Scab in Arcansas. In: Proceedings of National Fusarium Head Blight Forum, 26-27 October 1998 (pp 51–52) East Lansing, MI

    Google Scholar 

  • Moss MO and Frank JM (1985) Influence of the fungicide tridemorph on T-2 toxin production by Fusarium sporotrichoides. Transaction of the British Mycological Society 84: 585–590

    Google Scholar 

  • Munteanu I, Muresan T and Tataru V (1972) Fusarium wilt in wheat and integrated disease control in Romania. Acta Agronomica Academiae Scientiarum Hungaricae 21: 17–29

    Google Scholar 

  • Palti J (1978) Toxigenic Fusaria, their distribution and signifi-cance as cause of disease in animal and man. Acta Phytomedica 6: 110

    Google Scholar 

  • Parry DW, Jenkinson P and McLeod L (1995) Fusarium Ear Blight (scab) in small grain cereals - a review. Plant Pathology 44: 207–238

    Google Scholar 

  • Pirgozliev SR, Edwards SG, Hare MC and Jenkinson P (2001) Effect of timing and fungicides on the development of Fusarium Head Blight (FHB) and deoxynivalenol (DON) accumulation winter wheat. In: Proceedings of Sustainable systems of Cereal Crop Protection against Fungal Diseases and the Way of Reduction of Toxin Occurrence in Food Webs, 2-6 July 2001 (pp 178–182) Kromeriz, Czech Republic

    Google Scholar 

  • Pricket AJ, Macdonald S and Wildey KB (2000) Survey of Mycotoxins in Stored Grain from the 1999 Harvest in the UK. HGCA Project Report No 230. HGCA, London

    Google Scholar 

  • Ryu D and Bullerman LB (1999) Effect of cycling temperatures on the production of deoxynivalenol and zearalenone by Fusarium graminearum, NRRL 5883. Journal of Food Protection 62: 1451–1455

    Google Scholar 

  • Sayler T (1998) Study: $ 2.6 billion, 501 million bushels lost to scab 1991-1996. Prairie Grains 11

  • Simpson DR, Weston GE, Turner JA, Jennings P and Nicholson P (2001) Differential control of Head Blight pathogens of wheat by fungicides and consequences for mycotoxin contamination of grain. European Journal of Plant Pathology 104: 421–431

    Google Scholar 

  • Speers GM, Meronuck RA, Barnes DM and Mirocha CJ (1971) Effect of feeding Fusarium Roseum f.sp. graminearum contaminated corn and the mycotoxin F-2 on the growing chick and laying hen. Poultry Science 50: 627–633

    Google Scholar 

  • Suty A, Mauler-Machnik A and Courbon R (1996) New findings on the epidemiology of Fusarium Ear Blight on wheat and its control with tebuconazole. In: Proceedings of the Brighton Crop Protection Conference 1996: Pests and Diseases (pp 511–516) British Crop Protection Council, Farnham, United Kingdom

    Google Scholar 

  • Towers NR and Sprosen JM (1993) Zearalenone-induced infertility in sheep and cattle in NewZealand. New Zealand Veterinary Journal 41: 223–224

    Google Scholar 

  • Ueda S and Yoshizava T (1998) Effect of fungicides on abakabibyo and trichothecene production in wheat and barley. In: Proceedings of the Japanese Association of Mycotoxicology 28.Supplement 1. pp 234–235

    Google Scholar 

  • van Egmond HP (1989) Current situation on regulation for mycotoxins. Overview of tolerance and status of standard methods of sampling and analysis. Food Additives and Contaminants 6: 139–188

    Google Scholar 

  • Windels CE (2000) Economic and social impact of Fusarium Head Blight: changing farms and rural communities in the Northern Great Plains. Phytopathology 90: 17–21

    Google Scholar 

  • Yoshizava T (1983) Red-mold diseases and natural occurrence in Japan. In: Ueno Y (ed) Developments in Food Science-Trichothecenes: Chemical, Biological and Toxicological Aspects (pp 198–203) Tokyo, Kodansha

    Google Scholar 

  • Zadoks JC, Chang TT and Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Research 14: 415–421

    Google Scholar 

Download references

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Authors and Affiliations

  1. Crop and Environment Research Centre, Harper Adams University College, Newport, Shropshire, TF10 8NB, UK

    S.R. Pirgozliev, S.G. Edwards, M.C. Hare & P. Jenkinson

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  1. S.R. Pirgozliev
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  2. S.G. Edwards
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  3. M.C. Hare
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  4. P. Jenkinson
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Correspondence to P. Jenkinson.

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Pirgozliev, S., Edwards, S., Hare, M. et al. Effect of Dose Rate of Azoxystrobin and Metconazole on the Development of Fusarium Head Blight and the Accumulation of Deoxynivalenol (DON) in Wheat Grain. European Journal of Plant Pathology 108, 469–478 (2002). https://doi.org/10.1023/A:1016010812514

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