Abstract
Primary and clonal culture systems have been devised and refined for animal-derived satellite cells. Satellite cell (SC) culture development includes efficient cell isolation techniques, establishment of effective plating and growth conditions, formulation of media requirements and thorough evaluation of experimental limitations. As the field of muscle cell culture has expanded, the number of animal species from which satellite cells have been isolated has increased. The focus of this paper is to compare and contrast SC culture conditions presently used by a variety of researchers and to introduce a new source of SC from wapiti (elk).
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References
Allbrook DB, Han MF, Hellmuth AE (1971). Population of muscle satellite cells in relation to age and mitotic activity. Pathology 3(5): 223-245.
Allen RE (1987). Muscle cell culture as a tool in animal growth research. Fed Proc 46: 290-294.
Allen RE, Dodson MV, Luiten LS, Boxhorn LK (1985). A serum free medium that supports the growth of cultured skeletal muscle satellite cells. In Vitro Cell Dev Biol 21(11): 636-640.
Allen RE, Merkel RA, Young RB (1979). Cellular aspects of muscle growth: Myogenic cell proliferation. J Anim Sci 49: 115-127.
Allen RE, Sheeltan SM, Taylor RG, Kendall TL, Rice GM (1995). Hepatocyte growth factor activates quiescent skeletal muscle satellite cells in vitro. J Cell Physiol 165: 307-312.
Allen RE, Rankin LL, Greene EA, Boxhorn LK, Johnson SE, Taylor RG, Pierce PR (1991). Desmin is present in proliferating rat muscle satellite cells, but not in bovine muscle satellite cells. J Cell Physiol 149: 525-535.
Allen RE, Temm-Grove CJ, Sheehan SM (1995). Skeletal muscle satellite cell cultures. In: CP Emerson, HL Sweeney (eds), Methods In Cell Biology 52: 155-175.
Allen RE, Rankin LL (1990). Regulation of satellite cell during skeletal muscle growth and development. PSEMB 194: 81-86.
Anzil AP, Wernig A (1989). Muscle fiber loss and innervation after long term denervation. J Neurocyto 18(6): 833-845.
Baranska W, Baran W, Skopinski P, Ziemba H (1997). Quantitative ultrastructure evaluation of satellite cells in the soleus muscle from rats kept in hypokinesia. Exp Mol Path 64: 13-21.
Bischoff R (1974). Enzymatic liberation of myogenic cells from adult rat muscle. Anat Rec 180: 645-662.
Bischoff R (1986). Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol 115: 129-139.
Bischoff R (1989). Analysis of muscle regeneration using single myofibers in culture. Med Sci Sports Exerc 21(5): S164-S172.
Bischoff R (1994). The satellite cell and muscle regeneration. In: Engel AG, Franzini-Armstrong C (eds), Myology, pp 97-118.
Carlson BM, Faulkner JF (1983). The regeneration of skeletal muscle fibers following injury: A review. Med Sci Sports Exerc 15: 187-198.
Cantini M, Carraro U (1996). Control of cell proliferation by macrophage myoblast interactions. Basic Appl Myol 6(6): 485-489.
Carlson BM, Faulkner JF (1989). Muscle transplantation between young and old rats: Age of the host determines functional recovery. Am J Physiol 256: C1262-1266.
Carlson BM (1995). Factors influencing the repair and adaptation of muscles in aged individual: Satellite cells and innervation. J Geron Age 50A: 96-100.
Campion DR (1984). The satellite cell: A review. Int Rev Cyt 87: 225-251.
Church JC (1970). Cell populations in skeletal muscle after regeneration. J Embryol Exp Morphol 23(2): 531-537.
Cook DR, Doumit ME, Merkel RA (1993). TGF-Beta, basic FGF and platelet-derived growth factor-BB interact to affect proliferation of clonally derived porcine satellite cells. J Cell Physiol 157: 307-312.
Cornelison DW, Wold BJ (1997). Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells. Dev Biol 191: 270-283.
Dodson MV, McFarland DC, Martin EL, Brannon MA (1986). Isolation of satellite cells from ovine skeletal muscle. J Tiss Cul Meth 10(4): 233-237.
Dodson MV, Martin EL, Brannon MA, Mathison BA, McFarland DC (1987). Optimization of bovine satellite cell-derived myotube formation in vitro. Tissue and Cell 19(2): 159-166.
Dodson MV, Mathison BA (1988). Comparison of ovine and rat muscle-derived satellite cells: Response to insulin. Tissue and Cell 20(6): 909-918.
Dodson MV, Mathison BA, Mathison BD (1990). Effects of medium and substratum on ovine satellite cell attachment, proliferation and differentiation in vitro. Cell Diff Dev 29: 59-66.
Dodson, MV, Vierck JL, Hossner KL, Byrne K, McNamara JP (1997). The development and utility of a defined muscle and fat co-culture system. Tissue and Cell 29(5): 517-524.
Dodson MV, Vierck J, Velleman S, Koumans J (1995). Status of satellite cell research in agriculture. Basic Appl Myol 5: 5-10.
Dodson MV, McFarland DC, Grant AL, Doumit ME, Velleman SG (1996). Extrinsic regulation of domestic animal-derived satellite cells. Dom Anim Endocrin 13(2): 107-126.
Doumit ME, Merkel RA (1992). Conditions for the isolation and culture of porcine myogenic satellite cells. Tissue and Cell 24(2): 253-262.
Doumit Me, Cook DR, Merkel RA (1993). Fibroblast growth factor, epidermal growth factor, insulin-like growth factor and platelet-derived growth factor-BB stimulates proliferation of clonally derived porcine myogenic satellite cells. J Cell Physiol 157: 326-332.
Doumit ME, Cook DR, Merkel RA (1996). Testosterone up-regulates androgen receptors and decreases differentiation of porcine myogenic satellite cells in vitro. Endocrin 137(4): 1385-1394.
Doumit ME, McFarland DC, Minshall RD (1990). Satellite cells of growing turkeys: Influence of donor age and sex on proliferation and differentiation in vitro. Exp Cell Res 189: 81-86.
Doumit ME, Merkel RA (1991). Influence of IGF-I and insulin on proliferation and differentiation of porcine satellite cells. J Anim Sci 69(1): 316.
Drittani L, deBoland AR, Boland R (1989). Modulation of DNA synthesis in cultured muscle cells by 1,25-dihydroxy-vitamin D-3. Biochim Biophys Acta 1014: 112-119.
Duclos MJ, Chevalier B, Merchand-Brustel YL, Tanti JF, Goddard C, Simon J (1993). IGF-I stimulated glucose transport in myotubes derived from chicken muscle satellite cells. J Endocrin 137: 465-472.
Duclos MJ, Chevalier B, Goddard C, Simon J (1993). Regulation of amino acid transport and protein metabolism in myotubes derived from chicken muscle satellite cells by IGF-I. J Cell Physiol 157: 650-657.
Erickson S, Welter C, Calkins D, Vierck J, Krabbenhoft L, Byrne K, Greene E, Dodson MV (1998). Fusion of equine myogenic satellite cells in vitro. East Oragon J 14: 10-14.
Florini JR, Roberts SB (1979). A serum-free medium for the growth of muscle cells. In Vitro Cell Dev Biol 15: 983-992.
Floss T, Arnold HH, Braun T (1997). A role for FGF-6 in skeletal muscle regeneration. Genes Dev 11: 2040-2051.
Gal-Levi R, Leshem Y, Aoki S, Nakamura T, Halevy O (1998). Hepatocyte growth factor plays a dual role in regulating skeletal muscle satellite cell proliferation and differentiation. Biochem Biophys Acta 1402: 39-51.
Gospodarowicz D, Wesemaen J, Moran JS, Linstrom J (1976). Effects of FGF on the division and fusion of bovine myoblasts. J Cell Biol 70: 395-405.
Greene EA, Allen RE (1991). Growth factor regulation of bovine satellite cell growth in vitro. J Anim Sci 69: 146-152.
Greene EA, Raub RH (1992). Procedures for harvesting satellite cells from equine skeletal muscle. Equine Nut Phys Soc 12(1): 33-35.
Greene EA, Raub RH, Krabbenhoft EA, Brannon BA, Sullivan JM, Hossner KL, Dodson MV (1995). Initial observations on the growth factor regulation of equine satellite cells in vitro. Basic Appl Myol 5(1): 21-26.
Haigh JC, Hudson RJ (1993). Reinhardt R (ed), Farming Wapiti and Red Deer. Mosby Press.
Hathaway MR, Pampusch MS, Hembree JR, Dayton WR (1994). Transforming growth factor-Beta I facilitates established clonal populations of ovine muscle satellite cells. J Anim Sci 72: 2001-2007.
Hossner KL, Yemm R, Vierck J, Dodson MV (1997). Insulin-like growth factor (IGF)-I and-II and IGFBP secretion by ovine satellite cell strains grown alone or in coculture with 3T3-L1 preadipocytes. In Vitro Cell Dev Biol-Animal 33: 791-795.
Husmann I, Soulet L, Gautron J, Martelly I, Barritault D (1996). Growth factors in skeletal muscle regeneration. Cyto Growth Factor Rev 7(3): 249-258.
Jacobs SCJM, Wokke JHJ, Bar PR, Bootsma AL (1995). Satellite cell activation after damage in young and adult rats. Anat Rec 242: 329-336.
Johnson SE, Allen RE (1995). Activation of satellite cells and the role of fibroblast growth factor receptors. Exp Cell Res 219: 449-453.
Joubert Y, Tobin C (1995). Testosterone treatment results in quiescent satellite cells being activated and recruited into cell cycle in the rat levator ani muscle. Dev Biol 169: 286-294.
Katz, B (1961). Philosophic Transact Royal Soc London Ser B 243: 221-240
Koishi K, Zhang M, McLennan IS, Harris AJ (1995). Myo D protein accumulates in satellite cells and is nuerally regulated in regenerating myotubes and skeletal muscle fibers. Dev Dyn 202: 244-254.
Koumans LTM, Akster HA, Dulos GJ, Osse JWM (1990). Myosatellite cells of Cyprinus carpio (Teleostei) in vitro: isolation, recognition and differentiation. Cell Tissue Res 261: 173-181.
Lawson-Smith MJ, McGeachie JK (1997). Experimental skeletal muscle grafts as a model of regeneration. Aust NZ J Surg 67: 35-39.
Matschak TW, Stickland NC (1995). The growth of Atlantic salmon (Salmo salar L.) myosatellite cells in culture at two different temperatures. Experientia 51(3): 260-266.
Mauro A (1961). Satellite cells of skeletal muscle fibers. Biophys Biochem Cytol 9: 493-495.
McFarland DC, Doumit ME, Minshall RD (1988). The turkey myogenic satellite cell: Optimization of in vitro proliferation and differentiation. Tissue and Cell 20(6): 899-908.
McFarland DC, Pesall JE, Gilkerson KK (1993). The influence of growth factors on turkey embryonic myoblasts and satellite cells in vitro. Gen Comp Endocrin 89: 415-424.
McFarland DC, Pesall JE, Gilkerson KK, Ferrin NH (1991). Comparison of the proliferation and differentiation of myogenic satellite cells and embryonic myoblasts derived from the turkey. Comp Biochem Physiol 100A(2): 439-443.
McFarland DC, Gilkerson KK, Pesall JE, Ferrin WH, Wellenreiter RH (1997). In vitro characteristics of myogenic satellite cells derived from the pectoralis major and biceps femoris muscles of the chicken. Cytobios 91: 45-52.
McFarland DC, Pesall JE, Norberg JM, Dvoracek MA (1991). Proliferation of the Turkey myogenic satellite cell in serum-free medium. Comp Biochem Physiol 99A: 163-167.
McLennan IS, Koishi K (1997). Cellular localization of transforming growth factor-beta 2 and-beta 3 (TGF-beta2, TGF-beta3) in damaged and regenerating skeletal muscle. Dev Dyn 208: 278-289.
Minshall RD, McFarland DC, Doumit ME (1990). Interaction of insulin-like growth factor I with turkey satellite cells and satellite cell-derived myotubes. Dom Ani Endocrin 7(3): 413-424.
Molnar G, Dodson MV (1992). Characterization of sheep semimembranosus muscle and associated satellite cells: Expression of fast and slow myosin heavy chain. Basic Appl Myol 2(3): 183-190.
Molnar G, Dodson MV (1993). Satellite cells isolated from sheep skeletal muscle are not homogenous. Basic Appl Myol 3(3): 173-180.
Molnar G, Ho ML, Schroedl NA (1996). Evidence for multiple satellite cell populations and a non-myogenic cell type that is regulated differently in regenerating and growing skeletal muscle. Tissue & Cell 28(5): 547-556.
Moss FP, Leblond CP (1970). Nature of dividing nuclei in skeletal muscle of growing rats. J Cell Biol 44: 459-462.
Moss FP, Leblond CP (1971). Satellite cells as the source of nuclei in muscles of growing rats. Anat Rec 170: 421-435.
Mulvaney DR, Cyrino JEP (1995). Establishment of channel catfish satellite cell cultures. Basic Appl Myol 5(1): 65-70.
Ontell M (1974). Muscle satellite cells: a validated technique for light microscopic identification to quantify study of changes in their population following denervation. Anat Rec 178: 211-228.
Powell RL, Dodson MV, Cloud JG (1989). Cultivation and differentiation of satellite cells from skeletal muscle of the rainbow trout Salmo gairdner. J Exp Zool 250: 333-338.
Rantanen J, Hurme T, Lukka R, Heino J, Kalimo H (1995). Satellite cell proliferation and the expression of myogenin and desmin in regenerating skeletal muscle: Evidence for two different populations of satellite cells. Lab Invest 72(3): 341-347.
Ridpath JF, Huiatt TW, Trenkle AH, Robson RM, Bechtel PJ (1984). Growth and differentiation of chicken embryo muscle cell cultures derived from fast-and slow-growing lines: Intrinsic differences in growth characteristics and insulin response. Diff 26: 121-126.
Roe JA, Harper JMM, Buttery PJ, (1989). Protein metabolism in ovine primary muscle cultures derived from satellite cells-effects of selected peptide hormones and growth factors. J Endocrin 122: 565-571.
Rosenblatt JD, Lunt AI, Parry DJ, Partridge TA (1995). Culturing satellite cells from single muscle fiber explants. In Vitro Cell Dev Biol-Animal 31: 773-779.
Sadeh ME (1988). Effects of ageing on skeletal muscle regeneration. J Neurol Sci 87: 67-74.
Schultz E, Lipton BH (1982). Skeletal muscle Satellite cells: Changes in proliferation potential as a function of age. Mech Age Dev 20: 63-72.
Schultz E, McCormick KM (1994). Skeletal muscle satellite cells. Rev Phys Biochem Pharm 123: 213-257.
Soukup T, Thornell L (1997). Expression of myosin heavy chain isoforms in regenerated muscle spindle fibers after muscle grafting in young and adult rats-plasticity of intrafusal satellite cells. Diff 62: 179-186.
Stewart NT, Krabbenhoft EA, Foss M, Carraro U, Cantini M, Byrne K, Greene EA, Dodson MV (1996). Invited review: Clinical aspects of muscle regeneration: The satellite cell-macrophage connection. The Equine Athlete 9(6): 1, 7-11.
Stewart NT, Foss M, Carraro U, Cantini M, Byrne K, Vierck JL, Chen Y, Greene EA, Dodson MV (1997). Muscle regeneration is modulated by satellite cellmacrophage interactions at the site of muscle injury: Prospective clinical applications. J Equine Vet Sci 17(4): 172-177, 217-219.
Straus DS (1989). Regulation by insulin of cellular growth and proliferation: Relationship to the insulinlike growth factors. In: Draznim B, Melmed S, LeRoth, D (eds), Insulin Action, pp 143-152. New York: Alen R Liss, Inc., Publishers.
Strohman RC, Kardami F (1986). Muscle regeneration revisited: Growth factor regulation of myogenic cell replication. Prog Clin Biol Res 226: 287-296.
Teraux JP, Portalier P (1996). Cultures of striatal cholinergic neurons: Phenotypic changes in the presence of muscle fiber. Basic Appl Myol 6(6): 549-468.
Venkateswaran V, Brackett EJ, Vierck J, Cloud JG, and Dodson MV (1995). Substratum is an important determinant in growth factor regulation of troutderived satellite cells. Basic Appl Myol 5(3): 297-304.
Vierck JL, McNamara JP, Hossner KL, Dodson MV (1995). Characterizations of ovine skeletal muscle satellite cell strains in a defined culture medium formulated to enhance differentiation: Fusion and the IGF-I system. Basic Appl Myol 5(1): 11-20.
Vierck JL, Dodson MV (1999). Proper interpretation of cell culture phenomenon. Meth Cell Sci submitted.
Yablonka-Reuveni Z, Quinn LS, Nameroff M (1987). Isolation and clonal analysis of satellite cells from chicken pectoralis muscle. Dev Biol 119: 252-259.
Yu K, Czech MP (1984). The type I insulin-like receptor mediates the rapid effects of multiplicationstimulating activity on membrane transport systems in rat soleus muscle. J Biol Chem 259(5): 3090-3095.
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Burton, N.M., Vierck, J., Krabbenhoft, L. et al. Methods for animal satellite cell culture under a variety of conditions. Methods Cell Sci 22, 51–61 (2000). https://doi.org/10.1023/A:1009830114804
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DOI: https://doi.org/10.1023/A:1009830114804