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Effect of various cytokinins and auxins on in vitro regeneration of plantlets from isolated bud clumps of Silk Banana var. Nanjanagud Rasabale (Musa AAB)

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Abstract

Separated bud clumps of Silk Banana containing single and double buds were transferred on media containing six different cytokinins at three different levels and the proliferation capacity was determined. Proliferation, though occurred in single bud explants, the rate of multiplication was lower when compared with double bud explants, irrespective of the type of cytokinin used. Maximum shoot proliferation was observed in explants cultured on media supplemented with thidiazuron and 2-isopentenyl adenine (2-iP), and was significantly superior when combined with kinetin and BAP. Meta-topolin though reported to be highly efficient in improving multiplication in other banana varieties, no appreciable response was observed during present investigation. Use of NAA (2 mg l−1) was found to promote rooting process with superior root induction parameters and survival.

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Acknowledgments

AAW is thankful to the Department of Science and Technology, Government of India for providing financial assistance in the form of INSPIRE Fellowship (IF 10076).

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Author notes

  1. Ajit Arun Waman & Pooja Bohra

    Present address: Division of Horticulture and Forestry, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, 744 101, India

Authors and Affiliations

  1. Department of Horticulture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra Campus, Bengaluru, 560065, India

    Ajit Arun Waman, Pooja Bohra & B. N. Sathyanarayana

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  1. Ajit Arun Waman
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  2. Pooja Bohra
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  3. B. N. Sathyanarayana
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Correspondence to Ajit Arun Waman.

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Waman, A.A., Bohra, P. & Sathyanarayana, B.N. Effect of various cytokinins and auxins on in vitro regeneration of plantlets from isolated bud clumps of Silk Banana var. Nanjanagud Rasabale (Musa AAB). Ind J Plant Physiol. 21, 64–69 (2016). https://doi.org/10.1007/s40502-016-0204-9

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  • DOI: https://doi.org/10.1007/s40502-016-0204-9

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