Skip to main content
SpringerLink
Log in

Not all sugars are sweet for banana multiplication. In vitro multiplication, rooting, and acclimatization of banana as influenced by carbon source-concentration interactions

  • Micropropagation
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Carbon sources have been considered as one of the most important factors for in vitro multiplication of excised tissues. The type and concentration of sugars are known to influence the success of any in vitro protocol. In the present investigation, the effect of four carbon sources (glucose, fructose, mannitol, and sucrose) used to supplement Murashige and Skoog medium at three concentrations (1, 2, and 3%) was studied with respect to in vitro multiplication, in vitro rooting, and hardening in ‘Silk’ banana. Fructose (2%) followed by sucrose (3%) were found to be most the congenial carbon sources for obtaining the highest shoot multiplication rates. Growth parameters were also found to be superior in the fructose-containing media; however, it performed poorly in terms of root induction. Whereas, medium containing sucrose (3%) supported 100% root induction of in vitro-derived shoots. Plantlets multiplied initially on glucose-containing (1 and 3%) and sucrose-containing (3%) media exhibited superior growth parameters after secondary hardening. We conclude that a change in the rooting media could improve the number of successfully hardened plants under ex vitro conditions. To our knowledge, this is the first report describing the effect of sugars on hardening-related parameters in AAB genome of banana and apparently on any variety of banana.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.

Similar content being viewed by others

References

  • Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice. Elsevier, Amsterdam, The Netherlands, p 48

    Google Scholar 

  • Buah JN, Kawamitsu Y, Yonemori S, Hayashi M, Murayama S (2000) Effects of various carbon sources and their combinations on in vitro growth and photosynthesis of banana plantlets. Plant Prod Sci 3:392–397

    Article  Google Scholar 

  • Capellades M, Lemeus R, Debergh P (1991) Effect of sucrose on starch accumulation and rate of photosynthesis of Rosa cultivated in vitro. Plant Cell Tiss Organ Cult 25:21–26

    Article  Google Scholar 

  • Chun YS, Wang Y, Xu XF, Sun Y, Zhu LH, Han ZH (2008) Regeneration from leaf segments of in vitro–grown shoots of Malus baccata. N Z J Crop Hortic Sci 36:233–238

    Article  Google Scholar 

  • Conner AJ, Xinrun Z, Wooding AR (1993) Micropropagation of oca on a high sucrose medium promotes starch accumulation and plant establishment in soil. N Z J Crop Hortic Sci 21:91–93

    Article  CAS  Google Scholar 

  • Cuenca B, Vieitez AM (2000) Influence of carbon source on shoot multiplication and adventitious bud regeneration in in vitro beech culture. Plant Growth Regul 32:1–12

    Article  CAS  Google Scholar 

  • Gurel S, Gulsen Y (1998) The effects of different sucrose, agar and pH levels on in vitro shoot production of almond (Amygdalus communis L.). Turk J Bot 22:363–373

    Google Scholar 

  • Hanumantharaya BG, Sathyanarayana BN, Waman AA (2011) Reduced media salt concentration improves in vitro rooting in Indian pennywort. J Cell Tissue Res 11:2771–2774

    Google Scholar 

  • Hazarika BN (1999) Acclimatization of aseptically cultured Citrus plants for in vivo conditions. PhD Thesis submitted to Gauhati University, Guwahati, India

  • Hussein N (2012) Effects of nutrient media constituents on growth and development of banana (Musa spp.) shoot tips cultured in vitro. Afr J Biotechnol 11:9001–9006

    CAS  Google Scholar 

  • Jaime ATS (2004) The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers. Bragantia Campinas 63:165–177

    Article  Google Scholar 

  • Kadota M, Niimi Y (2004) Influences of carbon sources and their concentrations on shoot proliferation and rooting of ‘Hosui’ Japanese pear. Hortic Sci 39:1681–1683

    Google Scholar 

  • Khafagy SAA (2007) Effect of different carbon source concentrations on in vitro proliferation and rooting of Grande Naine banana plantlets. Egypt J Appl Sci 22:504–511

    Google Scholar 

  • Kozai T (1991) Micropropagation under photoautotrophic conditions. In: Debergh PC, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 447–469

    Chapter  Google Scholar 

  • Kumarswamy M, Sudipta KM, Balasubramanya S, Anuradha M (2010) Effect of different carbon sources on in vitro morphogenetic response of patchouli (Pogostemon cablin Benth.). J Phytol 2:11–17

    Google Scholar 

  • Lalonde S, Boles E, Hellman H, Barker L, Patrick JW, Frommer WB, Ward JW (1999) The dual function of sugar carriers: transport and sugar sensing. Plant Cell 11:707–726

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Langford PJ, Wainwright H (1987) Effect of sucrose concentration on the photosynthetic ability of rose shoots in vitro. Ann Bot 60:633–640

    CAS  Google Scholar 

  • Li B, Wolyn DJ (1997) Interactions of ancymidol with sucrose and α-napthaleneacetic acid in promoting asparagus (Asparagus officinalis L.) somatic embryogenesis. Plant Cell Rep 16:879–883

    Article  CAS  Google Scholar 

  • Madhulatha P, Kirubakaran SI, Sakthivel N (2006) Effects of carbon sources and auxins on in vitro propagation of banana. Biol Plant 50:782–784

    Article  CAS  Google Scholar 

  • Marino G, Bertazza G, Magnanini E, Altan AD (1993) Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot. Plant Cell Tiss Organ Cult 34:235–244

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nhut DT, Le BV, Fukai S, Tanaka M, Van TT (2001) Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum young stem culture. Plant Growth Regul 33:59–65

    Article  Google Scholar 

  • Owen HR, Wengerd D, Miller A (1991) Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal and medium storage method. Plant Cell Rep 10:583–586

    Article  PubMed  CAS  Google Scholar 

  • Premkumar A, Barceloâ-Munoz A, Quesada MA, Mercado JA, Pliego-Alfaro F (2003) Influence of sucrose concentration on in vitro rooting, growth, endogenous sugars and ex vitro survival of juvenile avocado. J Hortic Sci Biotechnol 78:46–50

    CAS  Google Scholar 

  • Romano A, Norohna C, Martins- Loucao MA (1995) Role of carbohydrates in micropropagation of cork oak. Plant Cell Tiss Organ Cult 40:159–167

    Article  CAS  Google Scholar 

  • Singh SK, Shymal MM (2001) Effect of media and physical factors on in vitro rooting in roses. Hortic J 14:91–97

    Google Scholar 

  • Singh HP, Uma S, Selvarajan R, Karihaloo JL (2011) Micropropagation for production of quality banana planting material in Asia-Pacific. Asia-Pacific Consortium on Agricultural Biotechnology (APCoAB), New Delhi, India

    Google Scholar 

  • Singha S, Oberly GH, Townsind EC (1987) Changes in nutrient composition and pH of the culture medium during in vitro shoot proliferation of crab apple and pear. Plant Cell Tiss Organ Cult 11:209–220

    Article  Google Scholar 

  • Son SH, Choi SM, Lee YH, Choi KB, Yun SR, Kim JK, Park HJ, Kwon OW, Noh EW, Seon JH, Park YG (2000) Large scale growth and taxane production in cell cultures of Taxus cuspidata (Japanese yew) using a novel bioreactor. Plant Cell Rep 19:628–633

    Article  CAS  Google Scholar 

  • Sridhar TM, Naidu CV (2011) Effect of different carbon sources on in vitro shoot regeneration of Solanum nigrum (Linn.)—an important antiulcer medicinal plant. J Phytol 3:78–82

    CAS  Google Scholar 

  • Sul IW, Korban SS (1998) Effects of media, carbon sources, and cytokinins on shoot organogenesis in the Christmas tree Pinus sylvestris L. J Hortic Sci Biotechnol 73:822–827

    CAS  Google Scholar 

  • Takayama S, Misawa M (1979) Differentiation of Lilium bulbscales grown in vitro: effect of various cultural conditions. Physiol Plant 46:184–190

    Article  CAS  Google Scholar 

  • Torpe TA (1980) Perspective in plant cell and tissue culture. In: Vasil IK (ed) Supplement IIA. Academic, New York

    Google Scholar 

  • Vora NC, Jasrai YT (2011) Effect of various carbon sources on in vitro shoot multiplication of banana. Phytomorphology 61:111–116

    Google Scholar 

  • Waman AA, Bohra P, Sathyanarayana BN, Gourish RK, Ashok TH (2014) Micropropagules can profitably save the choicest Silk banana from extinction. Proc Natl Acad Sci India Sect B Biol Sci. doi:10.1007/s40011-014-0349-0

    Google Scholar 

  • Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Review: role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40:2837–2849

    Article  PubMed  CAS  Google Scholar 

  • Zimmerman RH (1983) Factors affecting in vitro propagation of apple cuttings. Acta Hortic 31:171–178

    Google Scholar 

Download references

Acknowledgment

Waman Ajit Arun thanks the Department of Science and Technology, Government of India, for providing financial assistance in the form of an INSPIRE Fellowship (IF 10076).

Author information

Author notes

  1. Ajit Arun Waman & Pooja Bohra

    Present address: Division of Horticulture and Forestry, Central Island Agricultural Research Institute, Port Blair, 744101, Andaman and Nicobar Islands, India

Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Horticulture, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra Campus, Bengaluru, 560065, India

    Ajit Arun Waman, Pooja Bohra & B. N. Sathyanarayana

Authors
  1. Ajit Arun Waman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pooja Bohra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. N. Sathyanarayana
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ajit Arun Waman.

Additional information

Editor: John Foster

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Waman, A.A., Bohra, P. & Sathyanarayana, B.N. Not all sugars are sweet for banana multiplication. In vitro multiplication, rooting, and acclimatization of banana as influenced by carbon source-concentration interactions. In Vitro Cell.Dev.Biol.-Plant 50, 552–560 (2014). https://doi.org/10.1007/s11627-014-9623-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-014-9623-3

Keywords

Search

Navigation