Skip to main content
SpringerLink
Log in

Changes in growth, antioxidant defense system and major essential oils constituents of Pelargonium graveolens plant exposed to nano-scale silver and thidiazuron

  • Original Article
  • Published:
Indian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Application of signal molecules are very potential elicitors for production of plant secondary metabolites. This study was performed to elucidate the potential impacts of different concentrations of nano-silver (NS: 20, 40 and 80 mg l−1) particles and thidiazuron (TDZ: 50, 75 and 100 µM) and their combinations on variations of growth indices, contents of plastid pigments, antioxidant enzymes, viz., superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) activities, accumulation of hydrogen peroxide (H2O2) and production of essential oils (EOs) and its major constituents in P. graveolens. Results showed that plant growth parameters increased with increasing concentrations of both TDZ (up to 75 µM) and NS (up to 40 mg l−1). Combined treatments of TDZ and NS at all employed concentrations were better in improving growth indices and pigments content, except when applied at 100 µM TDZ + 80 mg l−1 NS. Application of TDZ75 +NS40 significantly reduced H2O2 content by 61.2 % than control. The highest specific activity of SOD and POX were observed at TDZ50 + NS40 and TDZ75 + NS40 treatments, respectively. In contrast, CAT activity was strongly increased both by TDZ50 and TDZ75 + NS80 treatments. The major EOs constituents were identified as citronellol (C), geraniol (G), cirtonellyl formate, iso-menthone, linalool and E-caryophyllene. EOs of P. graveolens plant under combined treatment of TDZ75 + NS40 possessed C/G ratio equivalent to one, indicating EOs with the best scent quality.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abeles, F. B., & Biles, C. L. (1991). Characterization of peroxidases in lignifying peach fruit endocarp. Journal of Plant Physiology, 95, 269–273.

    Article  CAS  Google Scholar 

  • Aebi, H. (1984). Catalase in vitro. Method Enzymology, 105, 121–176.

    Article  CAS  Google Scholar 

  • Aghajani, Z., Pourmeidani, A., & Ekhtiyari, R. (2013). Effect of nano-silver on stages of plant growth and yield and composition of essential of Thymus kotschyanus Boiss. & Hohen. African Journal of Agricultural Research, 8, 707–710.

    Google Scholar 

  • Bakkali, F., Averbeck, S., Averbeck, D., & Idoamar, M. (2008). Biological effects of essential oils—a review. Food and Chemical Toxicology, 46, 446–475.

    Article  CAS  PubMed  Google Scholar 

  • Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276–287.

    Article  CAS  PubMed  Google Scholar 

  • Farooqi, A. H. A., & Shukla, A. (1990). Utilization of plant growth regulators in aromatic plant production. Chromatography, 12, 152–157.

    Google Scholar 

  • Hafis, C., Romero-Puertas, M. C., Rio, L. A., Abdelly, C., & Sandalio, L. M. (2011). Antioxidative response of Hordeum maritimum L. to potassium deficiency. Acta Physiologiae Plantarum, 33, 193–202.

    Article  Google Scholar 

  • Harris, A. T., & Bali, R. (2008). Models for oral uptake of nanoparticles in consumer products. Journal of Nanoparticle Research, 10, 691–695.

    Article  CAS  Google Scholar 

  • Hatami, M., & Ghorbanpour, M. (2014). Defense enzymes activity and biochemical variations of Pelargonium zonale in response to nanosilver particles and dark storage. Turkish Journal of Biology, 38, 130–139.

    Article  CAS  Google Scholar 

  • Lei, Z., Mingyu, S., & Xiao, W. (2008). Antioxidant stress is promoted by nano-anatase in spinach chloroplasts under UV-B radiation. Biological Trace Element Research, 121, 69–79.

    Article  PubMed  Google Scholar 

  • Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. Method Enzymology, 148, 350–382.

    Article  CAS  Google Scholar 

  • Lu, C. M., Zhang, C. Y., Wen, J. Q., & Wu, G. R. (2002). Research on the effect of nanometer materials on germination and growth enhancement of Glycine max and its mechanism. Soybean Science, 21, 68–171.

    Google Scholar 

  • Mukherjee, M., & Mahapatra, A. (2009). Effect of coinage metal nanoparticles and zwitterionic surfactant on reduction of [Co(NH3)5Cl](NO3)2 by iron. Colloid Surface, 350, 1–7.

    Article  CAS  Google Scholar 

  • Mutui, T. M., Mibus, H., & Serek, M. (2005). Effects of thidiazuron, ethylene, abscisic acid and dark storage on leaf yellowing and rooting of Pelargonium cuttings. Journal of Horticultural Science and Biotechnology, 80, 543–550.

    CAS  Google Scholar 

  • Priyadarshini, S., Deepesh, B., Zaidi, M. G. H., Pardha-saradhi, P., Khanna, P. K., & Arora, S. (2012). Silver nanoparticle-mediated enhancement in growth and antioxidant status of Brassica juncea. Applied Biochemistry and Biotechnology, 167, 2225–2233.

    Article  Google Scholar 

  • Ram, P., Kumar, B., Naqvi, A. A., Verma, R. S., & Patra, N. K. (2005). Post-harvest storage effect on quantity and quality of rose-scented geranium (Pelargonium sp. cv. ‘Bourbon’) oil in Uttaranchal. Flavour and Fragrance Journal, 20, 666–668.

    Article  CAS  Google Scholar 

  • Rao, B. R. R. (2002). Biomass yield, essential oil yield and essential oil composition of rose-scented geranium (Pelargonium species) as influenced by row spacings and intercropping with cornmint (Mehtha arvensis L.f. piperascens Malinv. Ex Holmes). Industrial Crops and Products, 16, 133–144.

    Article  CAS  Google Scholar 

  • Reid, M. S., Wollenweber, B., & Serek, M. (2002). Carbon balance and ethylene in the postharvest life of flowering hibiscus. Postharvest Biology and Technology, 25, 227–233.

    Article  CAS  Google Scholar 

  • Scravoni, J., Vasconcellos, M. C., Valmorbida, J., Ferri, A. F., Marques, M. O. M., Ono, E. O., et al. (2007). The emerging nano-corporate paradigm: Nanotechnology and the transformation of nature, food and agri-food systems. International Journal of Sociology of Agriculture and Food, 15, 22–44.

    Google Scholar 

  • Strader, L. C., Beisner, E. R., & Bartel, B. (2009). Silver ions increase auxin efflux independently of effects on ethylene response. Plant Cell, 21, 3585–3590.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Swamy, K. N., & Rao, S. S. R. (2009). Effect of 24-epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens (L.) Herit. Russian Journal of Plant Physiology, l56, 616–620.

    Article  Google Scholar 

  • Van-Zyl, R. L., Seatlholo, S. T., Van-Vuuren, S. F., & Viljoen, A. M. (2006). The biological activities of 20 nature identical essential oil constituents. Journal of Essential Oil Research, 18, 129–133.

    Article  CAS  Google Scholar 

  • Velikova, V., Yordanov, I., & Edreva, A. (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Science, 151, 59–66.

    Article  CAS  Google Scholar 

  • Zavaleta-Mancera, H. A., Lopez-Delgado, H., Loza-Tavera, H., Mora-Herrera, M., Trevilla-Garcia, C., Vargas-Suarez, M., & Ougham, H. (2007). Cytokinin promotes catalase and ascorbate peroxidase activities and preserves the chloroplast integrity during dark senescence. Journal of Plant Physiology, 164, 1572–1582.

    Article  CAS  PubMed  Google Scholar 

  • Zheng, L., Hong, F., Lu, S., & Liu, C. (2005). Effect of nano-TiO2 on strength of naturally aged seeds and growth of spinach. Biological Trace Element Research, 104, 82–93.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, 38156-8-8349, Arak, Iran

    Mansour Ghorbanpour & Mehrnaz Hatami

Authors
  1. Mansour Ghorbanpour
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mehrnaz Hatami
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mansour Ghorbanpour.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ghorbanpour, M., Hatami, M. Changes in growth, antioxidant defense system and major essential oils constituents of Pelargonium graveolens plant exposed to nano-scale silver and thidiazuron. Ind J Plant Physiol. 20, 116–123 (2015). https://doi.org/10.1007/s40502-015-0145-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40502-015-0145-8

Keywords

Search

Navigation