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Deoxyribonucleic Acid Barcoding of Some Indian Coelogyninae (Orchidaceae)

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Subtribe Coelogyninae (Epidendroideae, Orchidaceae) comprises of 16 genera. The present study is undertaken to develop DNA barcodes of subtribe Coelogyninae using rbcL. It reveals the sectional delineation and its relationship with Pholidota. DNA barcoding is a technique which helps identification of species even if only small fragment of organism is available at any stage. The rbcL (Rubisco large subunit) from chloroplast genome is tested for effective barcoding. The interspecific divergence values and species discrimination rates were calculated by Kimura 2 parameter using MEGA 4.0. The rbcL with average interspecific divergence values yielded 72.72 % species resolution, thus it could distinguish all the species of Coelogyne and Pholidota. Invariably the genus Pholidota shows close affinity with Coelogyne. This supports the inclusion of genus Pholidota in the subtribe Coelogyninae of tribe Coelogyneae.

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Acknowledgments

Thanks are due to University Grants Commission for providing fellowship in the form of Rajiv Ghandi National Fellowship to J. Ramudu; also grateful to Dr. M. U, Sharief, Orchid Specialist, National Orchidarium and Experimental Garden, Yercaud (Tamil Nadu), India for identification of orchids.

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  1. Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, A.P., 522510, India

    S. M. Khasim & J. Ramudu

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  1. S. M. Khasim
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  2. J. Ramudu
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Correspondence to S. M. Khasim.

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Khasim, S.M., Ramudu, J. Deoxyribonucleic Acid Barcoding of Some Indian Coelogyninae (Orchidaceae). Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 277–284 (2018). https://doi.org/10.1007/s40011-016-0756-5

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