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Response of Datura innoxia Linn. to Gamma Rays and Its Impact on Plant Growth and Productivity

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Dry scarified seeds of Datura innoxia Linn. were gamma-irradiated with 5, 10, 20, 40, 60 and 80 Gy radiation, using a cobalt-60 source at the rate of 0.623 Gy min−1 at room temperature, and germinated on MS-medium in a growth chamber under controlled conditions. Exposure to low dose(s) of radiation (5 Gy) caused stimulatory effect on seed germination. Analysis of 90-day-old seedlings revealed that growth rate of root and shoot, net photosynthetic rate (P N ), stomatal conductance (gs) and the chlorophyll and carotenoid contents increased with 5 Gy radiations. Higher doses proved inhibitory for all the above parameters; the decline observed was positively correlated with increase in intensity of gamma radiation. Intercellular CO2 (Ci), on the other hand, showed an opposite trend, being lower with 5 Gy than in the control, but significantly higher with increased radiation doses. Hyoscyamine, a tropane alkaloid, exhibited only irregular and non-significant variation in its content, with no perceptible change in structure, in the treated material, indicating that gamma-irradiation caused no significant alteration in the quantity or quality of the compound.

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Acknowledgments

The authors thank Dr. Sumira Jan of the Centre for Research, University of Kashmir, Srinagar, for review of the text and Mr Zakir A Siddiqui of the Botany Department at Jamia Hamdard, New Delhi, for the help of drawings. They also appreciate the comments and suggestions of two unknown referees who reviewed this manuscript for PNAS, India.

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Authors and Affiliations

  1. Department of Plant Production, College of Food & Agricultural Sciences, King Saud University, PO Box 2460, Riyadh, 11451, Saudi Arabia

    Ibrahim M. Aref & Pervaiz R. Khan

  2. Department of Botany & Microbiology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia

    Abdulaziz A. Al Sahli

  3. Department of Biology, College of Natural and Computational Sciences, University of Gondar, P.O. Box 196, Gonder, Ethiopia

    Azamal Husen

  4. Department of Botany, Jamia Hamdard, Hamdard Nagar, Tughlaqabad, 110062, New Delhi, India

    M. K. A. Ansari,  Mahmooduzzafar & Muhammad Iqbal

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  1. Ibrahim M. Aref
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  2. Pervaiz R. Khan
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  3. Abdulaziz A. Al Sahli
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  7. Muhammad Iqbal
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Correspondence to Muhammad Iqbal.

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Aref, I.M., Khan, P.R., Al Sahli, A.A. et al. Response of Datura innoxia Linn. to Gamma Rays and Its Impact on Plant Growth and Productivity. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 623–629 (2016). https://doi.org/10.1007/s40011-014-0485-6

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  • DOI: https://doi.org/10.1007/s40011-014-0485-6

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