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Three dimensional reconstruction of bone-cartilage transitional structures based on semi-automatic registration and automatic segmentation of serial sections

  • Original Article
  • Tissue Engineering
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

A well-defined three-dimensional (3-D) reconstruction of bone-cartilage transitional structures is crucial for the osteochondral restoration. This paper presents an accurate, computationally efficient and semi-automated algorithm for the alignment and segmentation of two-dimensional (2-D) serial to construct the 3-D model of bonecartilage transitional structures. Entire system includes the following five components: (1) image harvest, (2) image registration, (3) image segmentation, (4) 3-D reconstruction and visualization, and (5) evaluation. A computer program was developed in the environment of Matlab for the semi-automatic alignment and automatic segmentation of serial sections. Semi-automatic alignment algorithm based on the position’s cross-correlation of the anatomical characteristic feature points of two sequential sections. A method combining an automatic segmentation and an image threshold processing was applied to capture the regions and structures of interest. SEM micrograph and 3-D model reconstructed directly in digital microscope were used to evaluate the reliability and accuracy of this strategy. The morphology of 3-D model constructed by serial sections is consistent with the results of SEM micrograph and 3-D model of digital microscope.

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Authors and Affiliations

  1. Department of Orthopaedics, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’ an, 710049, China

    Wei-Guo Bian

  2. Department of Orthopaedics, Xi’an Honghui Hospital Affiliated to Xi’an Jiaotong University, Xi’ an, 710049, China

    Hua Guo, Zheng-Wei Xu, Bao-Rong He & Ding-Jun Hao

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  1. Hua Guo
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  2. Zheng-Wei Xu
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  3. Bao-Rong He
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  4. Ding-Jun Hao
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  5. Wei-Guo Bian
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Correspondence to Wei-Guo Bian.

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Guo, H., Xu, ZW., He, BR. et al. Three dimensional reconstruction of bone-cartilage transitional structures based on semi-automatic registration and automatic segmentation of serial sections. Tissue Eng Regen Med 11, 387–396 (2014). https://doi.org/10.1007/s13770-014-0027-6

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  • DOI: https://doi.org/10.1007/s13770-014-0027-6

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