Abstract
So far, the production of bis-eugenol has been conducted by chemical synthesis. However, because chemical synthesis of bis-eugenol has disadvantages such as regioselectivity and low yield, an environmentally friendly production of bis-eugenol by biological synthesis has become attractive. In the present study, bis-eugenol was produced using Kalopanax pictus callus culture. High-performance liquid chromatography analysis of reaction product revealed a new peak, whose structure was determined to be bis-eugenol by nuclear magnetic resonance spectroscopy. After 18 h of biotransformation using K. pictus cell, 16.3 mg/L of biseugenol was produced without any byproducts. This is the first report on production of bis-eugenol by biotransformation using plant cell culture.
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B. G. Kim and J. Kim contributed equally.
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Kim, BG., Kim, J.Y., Yi, Y. et al. Biotransformation of eugenol to bis-eugenol by Kalopanax pictus cell culture. J Korean Soc Appl Biol Chem 55, 677–680 (2012). https://doi.org/10.1007/s13765-012-2105-6
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DOI: https://doi.org/10.1007/s13765-012-2105-6