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Non-covalently lactose imprinted polymers and recognition of saccharides in aqueous solutions

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Abstract

The aim of this work was to prepare lactose imprinted polymer and study of its selectivity for the recognition of different mono- and disaccharides. A series of molecularly imprinted polymers (MIPs) against lactose were synthesized and their binding properties were compared with a Blank non-imprinted polymer. Methacrylamide (MAAM) and ethylene glycol dimethacrylate were used as functional monomer and cross-linker, respectively. Dimethylsulfoxide was also applied as polymerization solvent. Different lactose:MAAM ratios were applied and optimized MIP was selected in a conventional batch adsorption study. The dissociation constant and maximum binding sites of polymer were determined using the Scatchard analysis. The selectivity of MIP for different mono- and disaccharides was also evaluated. The results indicated that the shape of cavity and orientation of functional monomers in binding sites and the spatial arrangement of hydroxyl groups in saccharide structure were responsible for the selectivity of lactose imprinted polymer.

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Abbreviations

AIBN:

2,2′-Azo-bis-iso-butyronitrile

BF:

Binding factor

B max :

Maximum binding sites

EGDMA:

Ethylene glycol dimethacrylate

K D :

Dissociation constant

MAAM:

Methacrylamide

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Acknowledgments

We gratefully acknowledge the Vice Chancellor of Research, Mashhad University of Medical Sciences for financial support through the grant number 88819. The results described in this paper were part of a PharmD student’s thesis.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Farzin Hadizadeh

  2. Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box: 91967-73117, Mashhad, Iran

    Amir Zakerian & Seyed Ahmad Mohajeri

Authors
  1. Farzin Hadizadeh
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  2. Amir Zakerian
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  3. Seyed Ahmad Mohajeri
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Correspondence to Seyed Ahmad Mohajeri.

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Hadizadeh, F., Zakerian, A. & Mohajeri, S.A. Non-covalently lactose imprinted polymers and recognition of saccharides in aqueous solutions. J IRAN CHEM SOC 10, 207–212 (2013). https://doi.org/10.1007/s13738-012-0142-z

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  • DOI: https://doi.org/10.1007/s13738-012-0142-z

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