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Development of an enzyme-free glucose sensor using the gate effect of a molecularly imprinted polymer

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Abstract

The instability of enzymatic glucose sensors has prevented the development of a practical artificial pancreas for diabetic patients. We therefore developed an enzyme-free glucose sensor using the gate effect of a molecularly imprinted polymer (MIP). This sensor has the advantages of improved stability and a simplified manufacturing procedure. An adduct of glucose and 4-vinylphenylboronic acid (VPBA) was synthesized by esterification and was then purified. The copolymer of the glucose/VPBA adduct and methylene bisacrylamide was grafted onto an indium tin oxide electrode surface. Glucose was washed out from the copolymer to obtain an MIP layer. Cyclic voltammetry of ferrocyanide in aqueous solution was performed using an MIP-grafted electrode, and the effect of glucose on the anodic current intensity was evaluated. The anodic current intensity was sensitive to the glucose concentration, and the dynamic range (0–900 mg/dl) covered the typical range of diabetic blood glucose levels. The response time of the MIP-grafted electrode to a stepwise change in the glucose concentration was approximately 3–5 min. Thus, we can conclude that, by taking advantage of its gate effect, it is feasible to use an MIP-grafted electrode as a glucose sensor for monitoring blood sugar in diabetic patients.

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References

  1. Unwin N, Marlin A. Diabetes action now: WHO and IDF working together to raise awareness worldwide. Diabetes Voice 2004;49: 27–31

    Google Scholar 

  2. Shichiri M, Yamazaki Y, Kawamori R, Hakui N, Abe H. Wearable artificial endocrine pancreas with needle-type glucose sensor. Lancet 1982;320: 1129–1131

    Article  Google Scholar 

  3. Shichiri M, Kishikawa H, Sakakida M, Kajiwara K, Hashiguchi Y, Nishida K, Uemura T, Konno Y, Ichinose K. Artificial endocrine pancreas and optimal blood glucose regulation in diabetic patients — from bedside type to wearable type. Diabetes Res Clin Pract 1994;24: S251–S259

    Article  PubMed  Google Scholar 

  4. Mastrototaro JJ, Cooper K, Shah R. Early clinical experience with an integrated continuous glucose sensor insulin pump platform. Diabetes Res Clin Pract 2006;74: S156–S159

    Article  CAS  Google Scholar 

  5. Ramström O, Yan M. Molecular imprinting — an introduction. In: Yan M, Ramström O (eds) Molecularly imprinted materials. New York: Marcel Dekker 2005;1–12

    Google Scholar 

  6. Haupt K. Molecularly imprinted polymers as recognition elements in sensors: mass and electrochemical sensors. In: Yan M, Ramström O (eds) Molecularly imprinted materials. New York: Marcel Dekker 2005;685–700

    Google Scholar 

  7. Vlatakis G, Andersson LI, Müller R, Mosbach K. Drug assay using antibody mimics made by molecular imprinting. Nature 1993;361: 645–647

    Article  CAS  PubMed  Google Scholar 

  8. Ulbricht M. Membrane separation using molecularly imprinted polymers. J Chromatogr B 2004;804: 113–125

    Article  CAS  Google Scholar 

  9. Sellergren B. Imprinted chiral stationary phase in high-performance liquid chromatography. J Chromatogr A 2001;906: 227–252

    Article  CAS  PubMed  Google Scholar 

  10. Piletsky SA, Piletskaya EV, Elgersma AV, Yano K, Karube I, Parhometz YP, El’skaya AV. Atrazine sensing by molecularly imprinted membranes. Biosens Bioelectron 1995;10: 959–964

    Article  CAS  Google Scholar 

  11. Piletsky SA, Piletskaya EV, Panasyuk TL, El’skaya AV, Levi R, Karube I, Wulff G. Imprinted membranes for sensor technology: opposite behavior of covalently and noncovalently imprinted membranes. Macromolecules 1998;31: 2137–2140

    Article  CAS  Google Scholar 

  12. Sergeyeva TA, Piletsky SA, Brovko AA, Slinchenko LA, Sergeeva LM, Panasyuk TL, El’skaya AV. Conductometric sensor for atrazine detection based on molecularly imprinted polymer membrane. Analyst 1999;124: 331–334

    Article  CAS  Google Scholar 

  13. Sergeyeva TA, Piletsky SA, Brovko AA, Slinchenko LA, Sergeeva LM, El’skaya AV. Selective recognition of atrazine detection by molecularly imprinted polymer membranes. Development of conductometric sensor for herbicides detection. Anal Chim Acta 1999;392: 105–111

    Article  CAS  Google Scholar 

  14. Yoshimi Y, Ohdaira R, Iiyama C, Sakai K. “Gate effect” of thin layer of molecularly-imprinted poly(methacrylic acid-coethyleneglycol dimethacrylate). Sens Actuators B Chem 2001;73: 49–53

    Article  Google Scholar 

  15. Sekine S, Watanabe Y, Yoshimi Y, Hattori K, Sakai K. Influences of solvents on chiral discriminative gate effect of molecularly imprinted poly(ethyleneglycol dimethacrylate-co-methacrylic acid). Sens Actuators B Chem 2007;127: 512–517

    Article  Google Scholar 

  16. Piletsky SA, Piletskaya EV, Yano K, Kugimiya A, Elgersma AV, Levi R, Kahlow U, Takeuchi T, Karube I. A biomimetic receptor system for sialic acid based on molecular imprinting. Anal Lett 1996;29: 157–170

    CAS  Google Scholar 

  17. Hattori K, Yoshimi Y, Sakai K. Gate effect of cellulosic dialysis membrane grafted with molecularly imprinted polymer. J Chem Eng Jpn 2001;34: 1466–1469

    Article  Google Scholar 

  18. Hattori K, Yoshimi Y, Ito T, Hirano K, Kohori F, Sakai K. Effect of electrostatic interactions on gate effect in molecularly imprinted polymers. Electrochemistry 2004;72: 508–516

    CAS  Google Scholar 

  19. Hattori K, Hiwatari M, Iiyama C, Yoshimi Y, Kohori F, Sakai K, Piletsky SA. Gate effect of theophylline-imprinted polymers grafted to the cellulose by living radical polymerization. J Membr Sci 2004;233: 169–173

    Article  CAS  Google Scholar 

  20. Wulff G. Selective binding to polymers via covalent bonds. The construction of chiral cavities as specific receptor sites. Pure Appl Chem 1982;54: 2093–2102

    Article  Google Scholar 

  21. Springsteen G, Wang B. A detailed examination of boronic acid-diol complexation. Tetrahedron 2002;58: 5291–5300

    Article  CAS  Google Scholar 

  22. Wulff G, Biffis A. Molecular imprinting with covalent or stoichiometric noncovalent interactions. In: Sellergren B (ed) Molecularly imprinted polymers: man-made mimics of antibodies and their applications in analytical chemistry. Amsterdam: Elsevier 2001;71–111

    Google Scholar 

  23. Kugimiya A, Yoneyama H, Takeuchi T. Sialic acid imprinted polymer-coated quartz crystal microbalance. Electroanalysis 2000;12: 1322–1326

    Article  CAS  Google Scholar 

  24. Bard AJ, Faulkner LR. Electrochemical methods: fundamentals and applications, 2nd edn. New York: Wiley 2001;226–260

    Google Scholar 

  25. Yoshimi Y, Haramoto H, Miyasaka T, Sakai K. Cathodic electrochemiluminescence of luminol enhanced by antibody-antigen reaction. J Chem Eng Jpn 1996;29: 851–857

    Article  CAS  Google Scholar 

  26. Iwata H, Matsuda T. ESCA — Surface analysis (I). In: Ikada Y (ed) Fundamental and Application of Polymer Surface (I). Kyoto: Kagaku Doujin 1986;89 (in Japanese)

    Google Scholar 

  27. American Diabetes Association Home Page, http://www.diabetes.org/about-diabetes.jsp. Accessed October 1, 2009

  28. Shapiro ET, Tillil H, Polonsky KS, Fang VS, Rubenstein AH, Van Cauter E. Oscillations in insulin secretion during constant glucose infusion in normal man: relationship to changes in plasma glucose. J Clin Endocrinol Metab 1988;67: 307–314

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Chemistry, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo, 135-8548, Japan

    Yasuo Yoshimi, Akisato Narimatsu, Keisuke Nakayama & Shinichi Sekine

  2. Department of Chemical Engineering, Waseda University, 3-7-5 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Koji Hattori & Kiyotaka Sakai

Authors
  1. Yasuo Yoshimi
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  2. Akisato Narimatsu
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  3. Keisuke Nakayama
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  4. Shinichi Sekine
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  5. Koji Hattori
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  6. Kiyotaka Sakai
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Correspondence to Yasuo Yoshimi.

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Yoshimi, Y., Narimatsu, A., Nakayama, K. et al. Development of an enzyme-free glucose sensor using the gate effect of a molecularly imprinted polymer. J Artif Organs 12, 264–270 (2009). https://doi.org/10.1007/s10047-009-0473-4

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  • DOI: https://doi.org/10.1007/s10047-009-0473-4

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