Skip to main content
Log in

A comparison of the use of thistle (Cynara cardunculus L.) and artichoke (Cynara scolymus L.) aqueous extracts for milk coagulation

  • Original Paper
  • Published:
Dairy Science & Technology

Abstract

Plant coagulants may offer a wide range of possibilities for cheese production, but before they can be used on an industrial scale, an in-depth study needs to be made concerning their suitability for cheese making and their effect on the coagulation process. The aims of this study were to determine the clotting activity of two plant coagulants in standard milk, to study the influence of different conditions (coagulant concentration, temperature, and frozen storage) on the coagulation of standard milk, and to compare the technological behavior of artichoke and thistle coagulants in goat milk. The coagulant activity of artichoke extract (46 IMCU mL−1) was lower than that of thistle extract (61 IMCU mL−1). Both extracts behaved similarly in the different conditions mentioned above in standard milk, and both can be used to curdle goat milk with no significant differences. The results point to similar rates of micellar aggregation for both plant coagulants, suggesting that artichoke could be used as an alternative coagulant to thistle for cheese making.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Castillo M (2001) Predicción del tiempo de corte en la elaboración de queso mediante dispersión de radiación de infrarrojo próximo. [Predicting cutting time in cheese making by dispersing near infrared radiation]. Ph. D. thesis. University of Murcia, Spain

    Google Scholar 

  • Castillo M, Payne FA, Hicks CL, López MB (2000) Predicting cutting and clotting time of coagulating goat’s milk using diffuse reflectance: effect of pH, temperature and enzyme concentration. Int Dairy J 10:551–562

    Article  CAS  Google Scholar 

  • Castillo M, Payne FA, González R, López MB, Laencina J (2003) Clotting time determination by NIR. An Vet Murcia 19:23–36

    Google Scholar 

  • Chazarra S, Sidrach L, López-Molina D, Rodríguez-López JN (2007) Characterization of the milk-clotting properties of extracts from artichoke (Cynara scolymus, L.) flowers. Int Dairy J 17:1393–1400

    Article  CAS  Google Scholar 

  • Dybowska E, Fujio Y (1996) Effect of temperature and glucono-δ-lactone GDL concentration on milk aggregation and gelation process as revealed by optical method. Milchwissenschaft 51:557–560

    CAS  Google Scholar 

  • Frazão C, Bento I, Costa J, Soares CM, Veríssimo P, Faro C, Pires E, Cooper J, Carrondo MA (1999) Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp-containing aspartic proteinase from the flowers of C. cardunculus L. J Biol Chem 274:27694–27701

    Article  Google Scholar 

  • García V, Rovira S, Teruel R, Boutoial K, Rodríguez J, Roa I, López MB (2012) Effect of vegetable coagulant, microbial coagulant and calf rennet on physicochemical, proteolysis, sensory and texture profiles of fresh goats cheese. Dairy Sci Technol 92:691–707

    Article  Google Scholar 

  • Hyslop DB, Richardson T, Ryans DS (1979) Kinetics of pepsin-initiated coagulation of κ-casein. Biochim Biophys Acta 556:390–396

    Article  Google Scholar 

  • IDF (2007) Milk. Determination of total milk-clotting activity of bovine rennets. Int Stand IDF 157. International Dairy Federation, Brussels

    Google Scholar 

  • Jacob M, Jaros D, Rohm H (2011) Recent advantages in milk clotting enzymes. Int J Dairy Technol 64:14–33

    Article  CAS  Google Scholar 

  • Kopelman IJ, Cogan U (1976) Determination of clotting power of milk clotting enzymes. J Dairy Sci 59:196–199

    Article  CAS  Google Scholar 

  • Llorente BE, Brutti CB, Caffini NO (2004) Purification and characterization of a milk-clotting aspartic proteinases from globe artichoke (Cynara scolymus L.). J Agric Food Chem 52:8182–8189

    Article  CAS  Google Scholar 

  • Llorente BE, Obregón WD, Avilés FX, Caffini NO, Vairo-Cavalli S (2014) Use of artichoke (Cynara scolymus) flower extract as a substitute for bovine rennet in the manufacture of Gouda-type cheese: characterization of aspartic proteases. Food Chem 159:55–63

    Article  CAS  Google Scholar 

  • Lo Piero AR, Puglisi I, Petrone G (2002) Characterization of “Lettucine”, a serine-like protease from Lactuca sativa leaves, as a novel enzyme for milk clotting. J Agric Food Chem 50:2439–2443

    Article  CAS  Google Scholar 

  • Mendiola FJ (2000) Obtención y caracterización del coagulante de leche normalizado de origen vegetal (Cynara cardunculus) y su aplicación en la elaboración de queso de La Serena. [Obtaining and characterization of plant standardized milk coagulant (Cynara cardunculus) and its application in the manufacturing of La Serena cheese]. Ph. D. thesis. University of Extremadura, Spain

  • Payens TAJ, Wiersma AK, Brinkhuis J (1977) On enzymatic clotting processes. I. Kinetics of enzyme-triggered coagulation reactions. Biophys Chem 6:253–262

    Article  CAS  Google Scholar 

  • Payne FA, Hicks CL (1992) Method for predicting cut-time of milk coagulum in cheese-making process. US Patent No. 5172,193

  • Pimentel C, Van Der Straeten D, Pires E, Faro C, Rodrigues-Pousada C (2007) Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. FEBS J 274:2523–2539

    Article  CAS  Google Scholar 

  • Raccuia SA, Mainolfi A, Mandolino G, Melilli MG (2004) Genetic diversity in Cynara cardunculus L. revealed by ALFP markers: comparison between cultivars and wild types of Sicily. Plant Breed 123:280–284

    Article  CAS  Google Scholar 

  • Roseiro LB, Barbosa M, Ames JM, Wilbey A (2003) Cheesemaking with vegetable coagulants—the use of Cynara L. for the production of ovine milk cheeses. Int J Dairy Technol 56:76–85

    Article  Google Scholar 

  • Sanjuán E, Millán R, Saavedra P, Carmona MA, Gómez R, Fernández-Salguero J (2002) Influence of animal and vegetable rennet on the physicochemical characteristics of Los Pedroches cheese during ripening. Food Chem 78:281–289

    Article  Google Scholar 

  • Sarmento AC, Lopes H, Oliveira CS, Vitorino R, Samyn B, Sergeant K, Debyser G, Van Beeumen J, Domingues P, Amado F, Pires E, Domingues MRM, Barros MT (2009) Multiplicity of aspartic proteinases from Cynara cardunculus L. Planta 230:429–439

    Article  CAS  Google Scholar 

  • Shah MA, Mir SA, Paray MA (2014) Plant proteases as milk-clotting enzymes in cheesemaking: a review. Dairy Sci Technol 94:5–16

    Article  CAS  Google Scholar 

  • Sidrach L, García-Cánovas F, Tudela J, Rodríguez-López JN (2005) Purification of cynarases from artichoke (Cynara scolymus L.): enzymatic properties of cynarase A. Phytochem 66:41–49

    Article  CAS  Google Scholar 

  • Silva SV, Malcata FX (2005) Studies pertaining to coagulant and proteolytic activities of plant proteases from Cynara cardunculus. Food Chem 89:19–26

  • Silva SV, Allmere T, Malcata FX, Andrén A (2003) Comparatives studies on the gelling properties of cardosins extracted from Cynara cardunculus and chymosin on cow's skim milk. Int Dairy J 13:559–564

    Article  CAS  Google Scholar 

  • Tabayehnejad N, Castillo M, Payne FA (2012) Comparison of total milk clotting activity measurement precision using the Berridge clotting time method and a proposed optical method. J Food Eng 108:549–556

    Article  Google Scholar 

  • Tavaria FK, Sousa MJ, Malcata FX (2001) Storage and lyophilization effects of extracts of Cynara cardunculus on the degradation of ovine and caprine caseins. Food Chem 72:79–88

    Article  CAS  Google Scholar 

  • van Hooydonk ACM, Walstra P (1987) Interpretation of the kinetics of the renneting reaction in milk. Neth Milk Dairy J 41:19–47

    Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Center for the Development of Industrial Technology (CDTI) under the Spanish Ministry of Science and Innovation. The company “Alimentos del Mediterraneo, S. Coop.” is also acknowledged.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to V. García.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

García, V., Rovira, S., Boutoial, K. et al. A comparison of the use of thistle (Cynara cardunculus L.) and artichoke (Cynara scolymus L.) aqueous extracts for milk coagulation. Dairy Sci. & Technol. 95, 197–208 (2015). https://doi.org/10.1007/s13594-014-0197-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13594-014-0197-y

Keywords

Navigation